| IED ID | IndEnz0002009937 |
| Enzyme Type ID | protease009937 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 16 EC 3.4.19.12 Deubiquitinating enzyme 16 AtUBP16 Ubiquitin thioesterase 16 Ubiquitin-specific-processing protease 16 |
| Gene Name | UBP16 At4g24560 F22K18.240 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MLLVLDLGISSLVLVVSLVLPLIGLFVRHKWRVAAQRREEIRRLLIHASEEAARAELEASVEFSSVAVSNVFHCPVCYCLATTRCSRCKAVRYCSGKCQIIHWRQGHKDECHPASIVYDSEDESDSDLRLGEENGQNTPEETLLVGPEPVTIPIGESLLSNRARSPEDGNGDIADNKDDLIDKEEAVSVAETSGSSFSGFSSSPRNDSGDEISRCESFSSSESERSESLLDAHVSVEPEDTCFSTIEDAPSKLLSPKFVHLVESVDNLANLPKLSVHKPEDDAGQNQSQSRSLHSLVTDRHPVSADPSLKSSDFWGTALGSAERVSDSCVKSKSGRPGNSSLHFSFGSGSSRDTSAAKVSEQRSSILKEAPRGTGYISDGVNLRERNAKRFDEAEIALPISSSTDALSPLDSSNLSHVTLPKSKSASSENGSMLAPLKVGEVQLLASKASNTKKCADLMKHSPLGAKSVRVLDHQKQNGAVVQHINSLHGRSGLKASVLKVVDQWTRPKSENEMAGRHGHKGLFPYEVFAKLYTYKIEFQPCGLINVGNSCFANVVFQCLMFTPPLTTYFLQQFHSRACTKKEQCFTCGFEKLVVKAKEEKSPLSPNGLLSQLQNIGIFLGNGKEEDAHEFLRFVVDTMQSVCIKASEYDMTKSSKLEDTTLIGLTFGGYLRSKIKCMKCQVKSELREKMMDLTVEIDGDISTLDDALRRFTRTEILDGENKYRCGSCKSYERAKKKLKITEPPNVLTIALKRFQAGKFGKLNKLIRFPETLDLAPYVSGGSEKSHDYKLYGVIVHLDVMNAAFSGHYVCYIRNQNKWYKADDSTVVTSDVERILTKGAYMLFYARCTPTPPRLAVCTKTEASNKKSRVPLPKANEKSTISRSVSTSSPELSSNTPGGGRSGNIQSFYSSFQRLQKILEEDSASDSSSLFDSNSDECSCSTDSTSMDDFADFIFGDHQGRAHGQSETPSPTSSSSSSSPPFTRRSPLSRSSPETYGTSRHQLPLGGER |
| Enzyme Length | 1008 |
| Uniprot Accession Number | Q9SB51 |
| Absorption | |
| Active Site | ACT_SITE 551; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10092; ACT_SITE 807; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10092 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:23232097}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Involved in salt tolerance by modulating sodium transport activity and repressing cell death at least partially through modulating SHM1 stability and activity (PubMed:23232097). Involved in cadmium tolerance by interacting with HIPP27 and probably modulating its stability (PubMed:23857362). {ECO:0000269|PubMed:23232097, ECO:0000269|PubMed:23857362}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (6); Domain (1); Metal binding (8); Mutagenesis (1); Region (7); Sequence conflict (1); Transmembrane (1); Zinc finger (1) |
| Keywords | Hydrolase;Membrane;Metal-binding;Protease;Reference proteome;Thiol protease;Transmembrane;Transmembrane helix;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | Q9SZJ5; O23254 |
| Induction | INDUCTION: Regulated by the transcription factors NAC045 and NAC086 and up-regulated by salt stress. {ECO:0000269|PubMed:23232097, ECO:0000269|PubMed:25081480}. |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18485060; 18650403; 18775970; 28695315; |
| Motif | |
| Gene Encoded By | |
| Mass | 110,600 |
| Kinetics | |
| Metal Binding | METAL 74; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 77; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 85; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 88; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 94; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 98; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 107; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134; METAL 111; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00134 |
| Rhea ID | |
| Cross Reference Brenda |