IED Industry Enzyme Database

Detail Information for IndEnz0002010011
IED ID IndEnz0002010011
Enzyme Type ID protease010011
Protein Name Reelin
EC 3.4.21.-
Reeler protein
Gene Name Reln Rl
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MERGCWAPRALVLAVLLLLATLRARAATGYYPRFSPFFFLCTHHGELEGDGEQGEVLISLHIAGNPTYYVPGQEYHVTISTSTFFDGLLVTGLYTSTSIQSSQSIGGSSAFGFGIMSDHQFGNQFMCSVVASHVSHLPTTNLSFVWIAPPAGTGCVNFMATATHRGQVIFKDALAQQLCEQGAPTEATAYSHLAEIHSDSVILRDDFDSYQQLELNPNIWVECSNCEMGEQCGTIMHGNAVTFCEPYGPRELTTTCLNTTTASVLQFSIGSGSCRFSYSDPSITVSYAKNNTADWIQLEKIRAPSNVSTVIHILYLPEEAKGESVQFQWKQDSLRVGEVYEACWALDNILVINSAHREVVLEDNLDPVDTGNWLFFPGATVKHSCQSDGNSIYFHGNEGSEFNFATTRDVDLSTEDIQEQWSEEFESQPTGWDILGAVVGADCGTVESGLSLVFLKDGERKLCTPYMDTTGYGNLRFYFVMGGICDPGVSHENDIILYAKIEGRKEHIALDTLTYSSYKVPSLVSVVINPELQTPATKFCLRQKSHQGYNRNVWAVDFFHVLPVLPSTMSHMIQFSINLGCGTHQPGNSVSLEFSTNHGRSWSLLHTECLPEICAGPHLPHSTVYSSENYSGWNRITIPLPNAALTRDTRIRWRQTGPILGNMWAIDNVYIGPSCLKFCSGRGQCTRHGCKCDPGFSGPACEMASQTFPMFISESFGSARLSSYHNFYSIRGAEVSFGCGVLASGKALVFNKDGRRQLITSFLDSSQSRFLQFTLRLGSKSVLSTCRAPDQPGEGVLLHYSYDNGITWKLLEHYSYVNYHEPRIISVELPDDARQFGIQFRWWQPYHSSQGEDVWAIDEIVMTSVLFNSISLDFTNLVEVTQSLGFYLGNVQPYCGHDWTLCFTGDSKLASSMRYVETQSMQIGASYMIQFSLVMGCGQKYTPHMDNQVKLEYSANHGLTWHLVQEECLPSMPSCQEFTSASIYHASEFTQWRRVTVVLPQKTWSGATRFRWSQSYYTAQDEWALDNIYIGQQCPNMCSGHGSCDHGVCRCDQGYQGTECHPEAALPSTIMSDFENPSSWESDWQEVIGGEVVKPEQGCGVVSSGSSLYFSKAGKRQLVSWDLDTSWVDFVQFYIQIGGESAACNKPDSREEGILLQYSNNGGIQWHLLAEMYFSDFSKPRFVYLELPAAAKTPCTRFRWWQPVFSGEDYDQWAVDDIIILSEKQKQVIPVVNPTLPQNFYEKPAFDYPMNQMSVWLMLANEGMAKNDSFCATTPSAMVFGKSDGDRFAVTRDLTLKPGYVLQFKLNIGCTSQFSSTAPVLLQYSHDAGMSWFLVKEGCFPASAGKGCEGNSRELSEPTVYYTGDFEEWTRITIAIPRSLASSKTRFRWIQESSSQKNVPPFGLDGVYISEPCPSYCSGHGDCISGVCFCDLGYTAAQGTCVSNTPNHSEMFDRFEGKLSPLWYKITGGQVGTGCGTLNDGRSLYFNGLGKREARTVPLDTRNIRLVQFYIQIGSKTSGITCIKPRARNEGLVVQYSNDNGILWHLLRELDFMSFLEPQIISIDLPREAKTPATAFRWWQPQHGKHSAQWALDDVLIGVNDSSQTGFQDKFDGSIDLQANWYRIQGGQVDIDCLSMDTALIFTENIGKPRYAETWDFHVSASSFLQFEMNMGCSKPFSGAHGIQLQYSLNNGKDWQLVTEECVPPTIGCVHYTESSTYTSERFQNWRRVTVYLPLATNSPRTRFRWIQTNYTVGADSWAIDNVILASGCPWMCSGRGICDSGRCVCDRGFGGPFCVPVVPLPSILKDDFNGNLHPDLWPEVYGAERGNLNGETIKSGTCLIFKGEGLRMLISRDLDCTNTMYVQFSLRFIAKGTPERSHSILLQFSVSGGVTWHLMDEFYFPQTTSILFINVPLPYGAQTNATRFRLWQPYNNGKKEEIWIIDDFIIDGNNLNNPVLLLDTFDFGPREDNWFFYPGGNIGLYCPYSSKGAPEEDSAMVFVSNEVGEHSITTRDLSVNENTIIQFEINVGCSTDSSSADPVRLEFSRDFGATWHLLLPLCYHSSSLVSSLCSTEHHPSSTYYAGTTQGWRREVVHFGKLHLCGSVRFRWYQGFYPAGSQPVTWAIDNVYIGPQCEEMCYGHGSCINGTKCICDPGYSGPTCKISTKNPDFLKDDFEGQLESDRFLLMSGGKPSRKCGILSSGNNLFFNEDGLRMLVTRDLDLSHARFVQFFMRLGCGKGVPDPRSQPVLLQYSLNGGLSWSLLQEFLFSNSSNVGRYIALEMPLKARSGSTRLRWWQPSENGHFYSPWVIDQILIGGNISGNTVLEDDFSTLDSRKWLLHPGGTKMPVCGSTGDALVFIEKASTRYVVTTDIAVNEDSFLQIDFAASCSVTDSCYAIELEYSVDLGLSWHPLVRDCLPTNVECSRYHLQRILVSDTFNKWTRITLPLPSYTRSQATRFRWHQPAPFDKQQTWAIDNVYIGDGCLDMCSGHGRCVQGSCVCDEQWGGLYCDEPETSLPTQLKDNFNRAPSNQNWLTVSGGKLSTVCGAVASGLALHFSGGCSRLLVTVDLNLTNAEFIQFYFMYGCLITPSNRNQGVLLEYSVNGGITWNLLMEIFYDQYSKPGFVNILLPPDAKEIATRFRWWQPRHDGLDQNDWAIDNVLISGSADQRTVMLDTFSSAPVPQHERSPADAGPVGRIAFEMFLEDKTSVNENWLFHDDCTVERFCDSPDGVMLCGSHDGREVYAVTHDLTPTENWIMQFKISVGCKVPEKIAQNQIHVQFSTDFGVSWSYLVPQCLPADPKCSGSVSQPSVFFPTEGWKRITYPLPESLTGNPVRFRFYQKYSDVQWAIDNFYLGPGCLDNCGGHGDCLKEQCICDPGYSGPNCYLTHSLKTFLKERFDSEEIKPDLWMSLEGGSTCTECGVLAENTALYFGGSTVRQAITQDLDLRGAKFLQYWGRIGSENNMTSCHRPVCRKEGVLLDFSTDGGITWTLLHEMDFQKYISVRHDYILLPEGALTNTTRLRWWQPFVISNGLVVSGVERAQWALDNILIGGAEINPSQLVDTFDDEGSSHEENWSFYPNAVRTAGFCGNPSFHLYWPNKKKDKTHNALSSRELIIQPGYMMQFKIVVGCEATSCGDLHSVMLEYTKDARSDSWQLVQTQCLPSSSNSIGCSPFQFHEATIYNAVNSSSWKRITIQLPDHVSSSATQFRWIQKGEETEKQSWAIDHVYIGEACPKLCSGHGYCTTGAVCICDESFQGDDCSVFSHELPSYIKDNFESARVTEANWETIQGGVIGSGCGQLAPYAHGDSLYFNGCQIRQAATKPLDLTRASKIMFVLQIGSPAQTDSCNSDLSGPHTVDKAVLLQYSVNNGITWHVIAQHQPKDFTQAQRVSYNVPLEARMKGVLLRWWQPRHNGTGHDQWALDHVEVVLVSTRKQNYMMNFSRQHGLRHFYNRRRRSLRRYP
Enzyme Length 3461
Uniprot Accession Number Q60841
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.21.-
Enzyme Function FUNCTION: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. {ECO:0000269|PubMed:10880573}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Alternative sequence (2); Beta strand (148); Chain (1); Disulfide bond (34); Domain (9); Erroneous initiation (1); Glycosylation (20); Helix (20); Metal binding (7); Mutagenesis (3); Repeat (16); Sequence conflict (13); Signal peptide (1); Turn (11)
Keywords 3D-structure;Alternative splicing;Calcium;Cell adhesion;Developmental protein;Disulfide bond;EGF-like domain;Extracellular matrix;Glycoprotein;Hydrolase;Metal-binding;Protease;Reference proteome;Repeat;Secreted;Serine protease;Signal;Zinc
Interact With Q924X6; Q14114; P98155
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
Modified Residue
Post Translational Modification PTM: N-glycosylated and to a lesser extent also O-glycosylated. {ECO:0000269|PubMed:17548821}.
Signal Peptide SIGNAL 1..26; /evidence=ECO:0000255
Structure 3D X-ray crystallography (5)
Cross Reference PDB 2DDU; 2E26; 3A7Q; 5B4X; 6A48;
Mapped Pubmed ID 10090720; 10192793; 10328933; 10328934; 10328935; 10363948; 10380917; 10380922; 10399933; 10403203; 10407039; 10421573; 10487203; 10498279; 10515259; 10557205; 10571240; 10594067; 10612399; 10648716; 10704405; 10716451; 10876649; 10939329; 10952903; 11050125; 11137154; 11163262; 11217851; 11226314; 11239428; 11239431; 11248103; 11312598; 11401448; 11472853; 11493521; 11516395; 11535289; 11579379; 11580894; 11597142; 11683995; 11719201; 11744223; 11745613; 11830577; 11856531; 11859196; 11891343; 11900467; 11920707; 11951052; 11982630; 12019323; 12091317; 12093079; 12122064; 12130536; 12130776; 12196578; 12205665; 12217965; 12223565; 12244214; 12244323; 12357430; 12376533; 12399118; 12426372; 12437591; 12451131; 12466851; 12467588; 12490540; 12526739; 12526740; 12533617; 12561075; 12584604; 12586425; 12586436; 12629176; 12629181; 12644247; 12668639; 12687690; 12687696; 12687699; 12699780; 12707415; 12714971; 12722105; 12724835; 12727448; 12764029; 12764039; 12764042; 12773624; 12832536; 12865130; 12902388; 12925587; 1331923; 1376466; 14069672; 14535959; 14570978; 14578885; 14604760; 14645539; 14648677; 14674845; 14681479; 14687546; 14704420; 14715136; 14715945; 14724251; 14750162; 14757526; 14758363; 14765123; 14973240; 14980731; 14985430; 14992814; 15013630; 15062102; 15080896; 15163631; 15175346; 15196778; 15219705; 15236237; 15238450; 15249135; 15255972; 15273690; 15342729; 15376319; 15385965; 15452856; 15459104; 15504324; 15519245; 15525772; 15548227; 15548577; 15570159; 1557392; 15590156; 15590913; 15590934; 15617678; 15632144; 15634788; 15655250; 1565842; 15671176; 15677725; 15703255; 15703280; 15718228; 15733084; 15858069; 15882580; 15896852; 15902206; 15932616; 15964824; 15987942; 16007083; 16041369; 16081247; 16113080; 16139369; 16148228; 16162939; 16168404; 16193498; 16207762; 16266828; 1627461; 16284245; 16324103; 16330018; 16344141; 16354676; 16376115; 16378758; 16387638; 16407979; 16410414; 16419078; 16432903; 16438943; 16438965; 16452688; 16466907; 16499749; 16499884; 16529723; 16537572; 16540508; 16540585; 16571605; 16580148; 16600913; 16602821; 16615126; 16641251; 1664786; 16680166; 16697663; 16769115; 16774856; 16774995; 16807322; 16814267; 16819978; 1686837; 16870734; 16873583; 16880397; 16901480; 16920270; 16957075; 16957084; 16964252; 16977475; 16980614; 16996039; 17021036; 17045962; 17067303; 17074040; 17108173; 17112676; 17120005; 17146780; 17148949; 17148950; 17167084; 17169463; 17173877; 17174287; 17182786; 17185001; 17189620; 17229826; 17301088; 17307331; 17314278; 17330141; 17360405; 17360583; 17376983; 17394141; 17442808; 17452454; 17462911; 17470284; 17482548; 17493606; 17494763; 17499872; 17504446; 17507397; 17507568; 17553006; 1760869; 17646384; 17651422; 17694053; 17698586; 17720534; 17728262; 17825270; 1783386; 17875933; 17881522; 17904250; 17913789; 17928452; 17959802; 18005957; 18024993; 18055048; 18086886; 18093172; 1812234; 18197264; 18199763; 18213587; 18215621; 18260139; 18272682; 18279306; 18329113; 18337425; 1838778; 18395191; 18448089; 18448648; 18455991; 18469343; 18477607; 18494254; 18506028; 18509043; 18514414; 18521945; 18547243; 18579678; 18596172; 18614579; 18625063; 18675252; 18729224; 18768805; 18778775; 18786638; 18794345; 18840685; 18842893; 18848628; 18957219; 18973587; 18989690; 19000783; 19008461; 19020026; 19020049; 19036983; 19037081; 19103804; 19129405; 19166810; 19167437; 19221860; 19230858; 19258013; 19272360; 19277610; 19332887; 19366679; 19366869; 19379776; 19389366; 19398467; 19409883; 19412957; 19515914; 19571125; 19571148; 19572151; 19633980; 19641124; 19657336; 19666823; 19686682; 19698788; 19710317; 19712103; 19735296; 19759535; 19796633; 19805234; 19805388; 19874786; 19923199; 19948739; 19948886; 20016109; 20040495; 20053714; 20053715; 20064940; 20067496; 20081193; 20089898; 20123976; 20145110; 20170731; 20223215; 20357114; 20372082; 20392955; 20399730; 20410112; 20410119; 20421250; 20431123; 20439316; 20503375; 20506362; 20566868; 20610746; 20610758; 20620871; 20667915; 20668538; 20681075; 20685998; 20685999; 20711475; 20713522; 20720102; 20809939; 20817919; 20825492; 20847150; 20847152; 20849864; 20865728; 20943929; 21039973; 21048135; 21077330; 21084626; 21111240; 21114965; 21172456; 21185282; 21214893; 21220099; 21226776; 21228166; 21266412; 21325502; 21331324; 21368052; 21419114; 21440680; 21460838; 21467208; 21491433; 21516100; 21530336; 21647369; 21664258; 21666133; 21677750; 21697387; 21697392; 21701912; 21768377; 21814183; 21821099; 21826226; 21852430; 21862448; 21865321; 21879738; 21925158; 21942094; 21976526; 21976703; 21991391; 22021915; 22046330; 22069190; 22090484; 22095278; 22103416; 22151329; 22161684; 22228756; 22246438; 22248045; 22256618; 22261923; 2230805; 22351047; 22351125; 22355639; 22361156; 22376909; 22378868; 22421355; 22440796; 22442076; 22461901; 22479190; 22479201; 22573669; 22578469; 22581782; 22586277; 22588081; 22610366; 22623674; 22627288; 22645310; 22649246; 22665518; 22678282; 22699903; 22724066; 22726835; 22761302; 22770513; 2280222; 22806269; 22817342; 22819337; 22821687; 22898830; 22906231; 22923088; 2293612; 22990595; 22999972; 23042739; 23056351; 23056358; 23082219; 23083738; 23097495; 23104248; 23147502; 23197722; 23209795; 23259060; 23292680; 23307637; 23318582; 23364326; 23404109; 23406282; 23414458; 23447624; 23499423; 23523356; 23610438; 23669529; 23700474; 23749873; 23752244; 23758727; 23765158; 23817546; 23828756; 23863479; 23916658; 23931996; 23968836; 23976984; 23988635; 24030152; 24036464; 24056077; 24115331; 24134921; 24154525; 24210661; 24210904; 24239537; 24313315; 24381170; 24403153; 24449842; 24469813; 24487590; 24491816; 24519818; 24539699; 24550175; 24584565; 24591110; 24595293; 24639535; 24718993; 24742460; 24759695; 24876378; 24892761; 24931760; 24952961; 24969133; 25079229; 25085881; 25119525; 25147501; 25182747; 25201528; 25245104; 25246510; 25255925; 25285789; 25308109; 25418135; 25467980; 25573715; 25604249; 25637488; 25637489; 25704077; 25760459; 25788693; 25834049; 25845740; 25864922; 25875176; 25892301; 25926459; 25937288; 25979088; 26060116; 26152694; 26224852; 26235615; 26269635; 26317415; 26371318; 26377473; 26386179; 26387718; 26387942; 26441085; 26457609; 26491063; 26499080; 26525675; 26526966; 26564256; 26620267; 26633882; 26635527; 26655900; 26657772; 26662625; 26671461; 26733804; 26739753; 26802325; 26893343; 26975851; 26980442; 27071537; 2707487; 27170656; 27184477; 27224923; 27252624; 27307241; 27320921; 27346785; 27364165; 27426965; 27586054; 27611684; 27624722; 27667971; 27726110; 27806288; 27866325; 27909010; 27915032; 28039900; 28071719; 28123028; 28127276; 28165338; 28174271; 28213441; 28272472; 28284905; 28419454; 28484035; 28572005; 28943410; 28981617; 29045729; 29049379; 29082311; 29127326; 29138282; 29222813; 29285798; 29420689; 29444425; 29691400; 29716946; 29748916; 29880879; 29901792; 29961574; 30001399; 30077770; 30084950; 30110642; 30127721; 30158644; 30241938; 30271915; 30329130; 30344048; 30377227; 30392794; 30394609; 30406178; 30413696; 30532694; 30597194; 30635555; 30689541; 30726732; 30811468; 30861578; 30864157; 31070778; 31079931; 31080058; 31097699; 31098770; 31122949; 31143101; 31185198; 31200100; 31230943; 31315048; 31399471; 31415124; 31430754; 31491533; 31521773; 31624071; 31665922; 31667489; 31676717; 31680851; 31693895; 31729356; 31749685; 31831824; 31845734; 32012381; 32065683; 32083308; 32161359; 32203496; 32253238; 32471461; 32492253; 32541003; 32571922; 32594343; 32610618; 32633719; 32652719; 32675279; 32718435; 32780108; 32787521; 32856318; 32913108; 32962021; 33020269; 33060132; 33084572; 33135045; 33299187; 33377147; 33388358; 33448039; 33462115; 33582152; 33723434; 33739968; 33845123; 33863910; 34146586; 34170322; 34333070; 34414407; 34639052; 34708032; 4644329; 5527378; 5957201; 6123371; 6383524; 6811784; 6960362; 7127145; 7478297; 7505209; 7506500; 7534196; 7605626; 7607678; 7620210; 7621749; 7623115; 7715721; 7719127; 7734734; 7748558; 7777557; 7787761; 7817781; 7818087; 7838424; 7851897; 7859091; 7887492; 7894155; 7942094; 7969746; 7972007; 8013566; 8038564; 8081873; 8326827; 8352721; 8374731; 8377223; 8394556; 8395631; 8431760; 8454207; 8662236; 8694291; 8774953; 8804731; 8817336; 8863127; 8915827; 8985248; 9004271; 9037414; 9049633; 9120058; 9136766; 9205121; 9223338; 9322161; 9337136; 9338784; 9338785; 9348346; 9364067; 9367432; 9381139; 9406921; 9519640; 9603370; 9651544; 9671664; 9698328; 9716537; 9728912; 9736652; 9742148; 9742149; 9799614; 9804938; 9811621; 9816727; 9822747; 9842772; 9845246; 9852586; 9883721; 9952412;
Motif
Gene Encoded By
Mass 387,495
Kinetics
Metal Binding METAL 2061; /note=Zinc 1; METAL 2074; /note=Zinc 1; METAL 2179; /note=Zinc 1; METAL 2264; /note=Zinc 1; METAL 2397; /note=Zinc 2; METAL 2399; /note=Zinc 2; METAL 2460; /note=Zinc 2
Rhea ID
Cross Reference Brenda