| IED ID |
IndEnz0002010015 |
| Enzyme Type ID |
protease010015 |
| Protein Name |
Transcription factor STP1
|
| Gene Name |
STP1 BAP1 SSY2 YDR463W D8035.7 |
| Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Ascomycota
saccharomyceta
Saccharomycotina (true yeasts)
Saccharomycetes
Saccharomycetales
Saccharomycetaceae
Saccharomyces
Saccharomyces cerevisiae (Baker's yeast)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
|
| Enzyme Sequence |
MPSTTLLFPQKHIRAIPGKIYAFFRELVSGVIISKPDLSHHYSCENATKEEGKDAADEEKTTTSLFPESNNIDRSLNGGCSVIPCSMDVSDLNTPISITLSPENRIKSEVNAKSLLGSRPEQDTGAPIKMSTGVTSSPLSPSGSTPEHSTKVLNNGEEEFICHYCDATFRIRGYLTRHIKKHAIEKAYHCPFFNSATPPDLRCHNSGGFSRRDTYKTHLKARHVLYPKGVKPQDRNKSSGHCAQCGEYFSTIENFVENHIESGDCKALPQGYTKKNEKRSGKLRKIKTSNGHSRFISTSQSVVEPKVLFNKDAVEAMTIVANNSSGNDIISKYGNNKLMLNSENFKVDIPKRKRKYIKKKQQQVSGSTVTTPEVATQNNQEVAPDEISSATIFSPFDTHLLEPVPSSSSESSAEVMFHGKQMKNFLIDINSFTNQQQQAQDNPSFLPLDIEQSSYDLSEDAMSYPIISTQSNRDCTQYDNTKISQILQSQLNPEYLSENHMRETQQYLNFYNDNFGSQF |
| Enzyme Length |
519 |
| Uniprot Accession Number |
Q00947 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Transcription factor involved in the regulation of gene expression in response to extracellular amino acid levels. Synthesized as latent cytoplasmic precursor, which, upon a signal initiated by the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor system, becomes proteolytically activated and relocates to the nucleus, where it induces the expression of SPS-sensor-regulated genes, including the amino-acid permeases AGP1, BAP2, BAP3 and GNP1. Binding to promoters is facilitated by DAL81. Involved in the repression of genes subject to nitrogen catabolite repression and genes involved in stress response. Negatively regulated by inner nuclear membrane proteins ASI1, ASI2 and ASI3, which prevent unprocessed precursor forms that escape cytoplasmic anchoring from inducing SPS-sensor-regulated genes. May be involved in pre-tRNA splicing. {ECO:0000269|PubMed:10648791, ECO:0000269|PubMed:15126393, ECO:0000269|PubMed:15197729, ECO:0000269|PubMed:15314160, ECO:0000269|PubMed:16400180, ECO:0000269|PubMed:16735580, ECO:0000269|PubMed:16977312, ECO:0000269|PubMed:17085444, ECO:0000269|PubMed:1803818, ECO:0000269|PubMed:3072476, ECO:0000269|PubMed:9065387}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Compositional bias (3); Erroneous initiation (1); Mutagenesis (5); Propeptide (1); Region (5); Zinc finger (3) |
| Keywords |
Cell membrane;DNA-binding;Membrane;Metal-binding;Nucleus;Phosphoprotein;Reference proteome;Repeat;Zinc;Zinc-finger;Zymogen;tRNA processing |
| Interact With |
|
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=Localizes to the cytoplasm in its unprocessed form and is targeted to the nucleus after proteolytic processing upon induction by amino acids. The SCF(MET30) ubiquitin ligase complex negatively regulates nuclear accumulation of the processed form in the absence of high extracellular methionine levels. |
| Modified Residue |
|
| Post Translational Modification |
PTM: Phosphorylated by casein kinase I. Phosphorylation is not dependent on the extracellular amino acid levels, but is a prerequisite for proteolytic processing. {ECO:0000269|PubMed:12502738, ECO:0000269|PubMed:15509782}.; PTM: Activated by the amino acid-induced proteolytic removal of an N-terminal inhibitory domain by serine protease SSY5, an intrinsic component of the SPS-sensor. Processing requires at least 2 components of the SCF(GRR1) ubiquitin ligase complex, namely the F-box protein GRR1 and the E2 enzyme CDC34, but does not depend on the proteasome. Processing is negatively regulated by the protein phosphatase 2A regulatory subunit RTS1. |
| Signal Peptide |
|
| Structure 3D |
|
| Cross Reference PDB |
- |
| Mapped Pubmed ID |
10688190;
12125055;
12702263;
14690591;
15450611;
15667321;
16077013;
16554755;
17828247;
18307103;
18708352;
18951365;
19143640;
19494127;
19536198;
19635936;
19805573;
19882662;
19906648;
20041210;
20421414;
20675578;
21127045;
21179020;
21521246;
22189060;
22419079;
23358190;
23828602;
24260565;
24483210;
25218923;
25253722;
25825491;
26457198;
26546823;
26760377;
26825800;
2694676;
27656112;
27683589;
9483800;
9822825;
|
| Motif |
|
| Gene Encoded By |
|
| Mass |
58,088 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
|