| IED ID | IndEnz0002010026 |
| Enzyme Type ID | protease010026 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 36 EC 3.4.19.12 Deubiquitinating enzyme 36 Protein scrawny Ubiquitin thioesterase 36 Ubiquitin-specific-processing protease 36 |
| Gene Name | Usp36 scny GF23992 |
| Organism | Drosophila ananassae (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group ananassae subgroup ananassae species complex Drosophila ananassae (Fruit fly) |
| Enzyme Sequence | MPVSVAVCETTNVVNAALRESLGVGIGSGGASSDDKSAGEDTNSLQNHIVANAKRILMTKIEYEEVPNYQEAVLENLKSKYIVIKPTNPTNGCNLNGNTANTFGNKNNAGKIVGANGHDNNGRKLSDHPNQNHNHANPNGHHANPNELPKPKRVLYPRENIRIGWKQSERKWQVGSGMINAGNTCYLNSTLQALFHIPALANWLVSEQAHMENCNVSESGSFCIICAMAKTLQATQTTQSAVRPFLIYTKLKQICKHMIVGRQEDAHEFLRFLVEAMERAYLMRFRNYKELDQLVKETTPLGQIFGGYLRSEVRCLSCNHVSITFQHFQDLLLDIRKSDSLEEAFEGYFSREKLEDFGYKCEGCKKKVSATKQFRLERAPITLCIQLKRFSMMGNKLTKQITFKPRIDLSKFAARSPAASVQPLIYRLVSMVTHLGVSQHCGHYTAIGSTEAGSYYNFDDSYVRPIAIQSVCNTNAYIMFYELDPLQTSSPAAARANGLRLTNGHGPVPVAVPATVSSPLPSPAKFIGPQLPPGGINGYSNGHGPKTTIQFKPQHQPSHQQNGVQQSAKSPLLSTHVKVEAAAGAAALAASAAPTANGNKSSSNHSNHKSVNQQHYLPISSEDEDSEDEVKARPTVQLPSMPKMDDCMDSGKPKSPVKTPVKTPLKSLVPYESASEEEEVVPLPNPNARKRSSDSSDSEHEPTTSSVQLNGHSKTNGSLSNGSSKSTDAIDEIFKSLKGYQAKKKSADSEDDDDDEDEPNNQLTNGWHPQKQSQSQSRSGPPSPKTPPSPAVIKSKTGIWKVTRDDGDDDEDDDDDDDEVVEEARAVRTPVKNHRNPFASSKTATDSPTTPGAKRQKLLNGSAIKTQQQPRAGNGYQSEATANGGTVNELLKQSHRGYSSSVLSWNGKPAELEKEPFVLVCAKRIAGHGSLDGSGSGSNTDIIDTEIPAAAVNFPSGSCSFSLLADARDQRQRDLADDEENEMDRGRQRKVKSGSAKISNSTPGYNPFMEFENQKRWHKNGGGGGFPRFYQNQNFRQGFQQRNKFKFNRFGGPGSAKFQQQRALQRHLAAGGGFTRRQPTHSAQQQQQQQS |
| Enzyme Length | 1091 |
| Uniprot Accession Number | B3M3M6 |
| Absorption | |
| Active Site | ACT_SITE 185; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 443; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Controls selective autophagy activation by ubiquitinated proteins. {ECO:0000250|UniProtKB:Q9VRP5}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (9); Domain (1); Modified residue (13); Region (5) |
| Keywords | Hydrolase;Nucleus;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}. |
| Modified Residue | MOD_RES 518; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 522; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 659; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 663; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 673; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 675; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 749; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 783; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 786; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 789; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 829; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 847; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 850; /note=Phosphothreonine; /evidence=ECO:0000250 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 119,512 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |