| IED ID | IndEnz0002010037 |
| Enzyme Type ID | protease010037 |
| Protein Name |
UBX domain-containing protein 1 SAPK substrate protein 1 UBA/UBX 33.3 kDa protein |
| Gene Name | UBXN1 SAKS1 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MAELTALESLIEMGFPRGRAEKALALTGNQGIEAAMDWLMEHEDDPDVDEPLETPLGHILGREPTSSEQGGLEGSGSAAGEGKPALSEEERQEQTKRMLELVAQKQREREEREEREALERERQRRRQGQELSAARQRLQEDEMRRAAEERRREKAEELAARQRVREKIERDKAERAKKYGGSVGSQPPPVAPEPGPVPSSPSQEPPTKREYDQCRIQVRLPDGTSLTQTFRAREQLAAVRLYVELHRGEELGGGQDPVQLLSGFPRRAFSEADMERPLQELGLVPSAVLIVAKKCPS |
| Enzyme Length | 297 |
| Uniprot Accession Number | Q04323 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Ubiquitin-binding protein that plays a role in the modulation of innate immune response. Blocks both the RIG-I-like receptors (RLR) and NF-kappa-B pathways. Following viral infection, UBXN1 is induced and recruited to the RLR component MAVS. In turn, interferes with MAVS oligomerization, and disrupts the MAVS/TRAF3/TRAF6 signalosome. This function probably serves as a brake to prevent excessive RLR signaling (PubMed:23545497). Interferes with the TNFalpha-triggered NF-kappa-B pathway by interacting with cellular inhibitors of apoptosis proteins (cIAPs) and thereby inhibiting their recruitment to TNFR1 (PubMed:25681446). Prevents also the activation of NF-kappa-B by associating with CUL1 and thus inhibiting NF-kappa-B inhibitor alpha/NFKBIA degradation that remains bound to NF-kappa-B (PubMed:28152074). Interacts with the BRCA1-BARD1 heterodimer and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1 leads to the inhibition of the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer (PubMed:20351172). Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. {ECO:0000269|PubMed:20351172, ECO:0000269|PubMed:23545497, ECO:0000269|PubMed:25681446, ECO:0000269|PubMed:28152074}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (1); Chain (1); Coiled coil (1); Compositional bias (2); Domain (2); Initiator methionine (1); Modified residue (6); Mutagenesis (2); Natural variant (1); Region (2); Sequence conflict (3) |
| Keywords | Acetylation;Alternative splicing;Coiled coil;Cytoplasm;Direct protein sequencing;Host-virus interaction;Phosphoprotein;Reference proteome |
| Interact With | V9HW80; Q9Y263; Q9HCM9; Q7KZS0; Q9UMX0; Q9UMX0-2; Q8WU02; O00154-4; Q14203-5; Q08426; P04792; Q9UBY9; O60333-2; P02545; Q9Y263; Q9BUI4; O60260-5; P54725; Q9Y3C5; P37840; Q9UHD9; O76024 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23545497}. |
| Modified Residue | MOD_RES 2; /note="N-acetylalanine"; /evidence="ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:25944712"; MOD_RES 199; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:18669648"; MOD_RES 200; /note="Phosphoserine; by MAPK12"; /evidence="ECO:0000269|PubMed:15362974"; MOD_RES 207; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:24275569"; MOD_RES 229; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:23186163"; MOD_RES 270; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:24275569" |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15215856; 15358861; 15944415; 16055502; 16168377; 17353931; 18847512; 19367725; 19615732; 20195357; 21135095; 25416956; 26611529; 27785701; 29685906; 33754075; 34246306; |
| Motif | |
| Gene Encoded By | |
| Mass | 33,325 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |