| IED ID | IndEnz0002010056 |
| Enzyme Type ID | protease010056 |
| Protein Name |
Intestinal-type alkaline phosphatase IAP Intestinal alkaline phosphatase EC 3.1.3.1 |
| Gene Name | ALPI |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MQGPWVLLLLGLRLQLSLGVIPAEEENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGDTKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGGYTLRGSSIFGLAPSKAQDSKAYTSILYGNGPGYVFNSGVRPDVNESESGSPDYQQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPACTTDAAHPVAASLPLLAGTLLLLGASAAP |
| Enzyme Length | 528 |
| Uniprot Accession Number | P09923 |
| Absorption | |
| Active Site | ACT_SITE 111; /note=Phosphoserine intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10042 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a phosphate monoester + H2O = an alcohol + phosphate; Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879, ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.1; Evidence={ECO:0000250|UniProtKB:P15693, ECO:0000255|PROSITE-ProRule:PRU10042}; |
| DNA Binding | |
| EC Number | 3.1.3.1 |
| Enzyme Function | FUNCTION: Alkaline phosphatase that can hydrolyze various phosphate compounds. {ECO:0000250|UniProtKB:P15693}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (2); Glycosylation (3); Lipidation (1); Metal binding (14); Natural variant (2); Propeptide (1); Sequence conflict (3); Signal peptide (1) |
| Keywords | Calcium;Cell membrane;Direct protein sequencing;Disulfide bond;GPI-anchor;Glycoprotein;Hydrolase;Lipoprotein;Magnesium;Membrane;Metal-binding;Reference proteome;Signal;Zinc |
| Interact With | P60410; P60411; Q7Z3S9 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P15693}; Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:P15693}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence="ECO:0000269|PubMed:1458595, ECO:0000269|PubMed:3458202" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11072085; 12372831; 12919939; 14595869; 15143152; 15715171; 15774940; 15831710; 17353931; 17498884; 18292227; 18307834; 19407215; 19570815; 20489044; 22157815; 22262031; 22333159; 22783049; 24019514; 24043717; 24247025; 24296104; 2443973; 24856042; 24966474; 25037223; 25277244; 25409280; 25416956; 25609649; 25906119; 26299896; 26496610; 26844282; 26988336; 27083970; 27106638; 27384524; 27865621; 28931466; 29970708; 30417313; 31915470; 32472757; 32728117; 32906115; 32918806; 32934082; 33391458; 3422494; 34831233; |
| Motif | |
| Gene Encoded By | |
| Mass | 56,812 |
| Kinetics | |
| Metal Binding | METAL 61; /note=Magnesium; /evidence=ECO:0000250|UniProtKB:P15693; METAL 61; /note=Zinc 1; /evidence=ECO:0000250|UniProtKB:P15693; METAL 111; /note=Zinc 1; /evidence=ECO:0000250|UniProtKB:P15693; METAL 174; /note=Magnesium; /evidence=ECO:0000250|UniProtKB:P15693; METAL 235; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P05186; METAL 288; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:P05186; METAL 289; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P05186; METAL 304; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P05186; METAL 330; /note=Magnesium; /evidence=ECO:0000250|UniProtKB:P15693; METAL 335; /note=Zinc 2; /evidence=ECO:0000250|UniProtKB:P15693; METAL 339; /note=Zinc 2; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:P15693; METAL 376; /note=Zinc 1; /evidence=ECO:0000250|UniProtKB:P15693; METAL 377; /note=Zinc 1; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:P15693; METAL 451; /note=Zinc 2; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:P15693 |
| Rhea ID | RHEA:15017 |
| Cross Reference Brenda |