| IED ID | IndEnz0002010068 |
| Enzyme Type ID | protease010068 |
| Protein Name |
Transcriptional regulator URE2 Disulfide reductase EC 1.8.4.- Glutathione peroxidase EC 1.11.1.9 |
| Gene Name | URE2 YNL229C N1165 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MMNNNGNQVSNLSNALRQVNIGNRNSNTTTDQSNINFEFSTGVNNNNNNNSSSNNNNVQNNNSGRNGSQNNDNENNIKNTLEQHRQQQQAFSDMSHVEYSRITKFFQEQPLEGYTLFSHRSAPNGFKVAIVLSELGFHYNTIFLDFNLGEHRAPEFVSVNPNARVPALIDHGMDNLSIWESGAILLHLVNKYYKETGNPLLWSDDLADQSQINAWLFFQTSGHAPMIGQALHFRYFHSQKIASAVERYTDEVRRVYGVVEMALAERREALVMELDTENAAAYSAGTTPMSQSRFFDYPVWLVGDKLTIADLAFVPWNNVVDRIGINIKIEFPEVYKWTKHMMRRPAVIKALRGE |
| Enzyme Length | 354 |
| Uniprot Accession Number | P23202 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 124; /note=Glutathione; /evidence=ECO:0000269|PubMed:11695904; BINDING 151; /note=Glutathione; /evidence=ECO:0000269|PubMed:11695904 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=2 glutathione + H2O2 = glutathione disulfide + 2 H2O; Xref=Rhea:RHEA:16833, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240, ChEBI:CHEBI:57925, ChEBI:CHEBI:58297; EC=1.11.1.9; Evidence={ECO:0000269|PubMed:19321443}; |
| DNA Binding | |
| EC Number | 1.8.4.-; 1.11.1.9 |
| Enzyme Function | FUNCTION: Plays an important role in nitrogen catabolite repression. Down-regulates the expression of many genes involved in nitrogen utilization by inhibiting the GATA transcriptional activators GLN3 and GAT1. Under good nitrogen conditions, binds to the phosphorylated forms of GLN3 and GAT1 and sequesters them in the cytoplasm, preventing transcription of genes expressed upon nitrogen limitation. Is also an atypical glutaredoxin without a catalytical cysteine residue. Has glutathione peroxidase and thiol:disulfide oxidoreductase activities in both native and fibrillar form. Also shows insulin disulfide reductase and dehydroascorbic acid reductase (DHAR) actvites. {ECO:0000269|PubMed:10604478, ECO:0000269|PubMed:10799523, ECO:0000269|PubMed:15371425, ECO:0000269|PubMed:19321443, ECO:0000269|PubMed:1990286, ECO:0000269|PubMed:8755910}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (6); Binding site (2); Chain (1); Domain (2); Helix (17); Initiator methionine (1); Modified residue (1); Mutagenesis (3); Region (4); Turn (3) |
| Keywords | 3D-structure;Acetylation;Amyloid;Cytoplasm;Nitrate assimilation;Oxidoreductase;Prion;Reference proteome |
| Interact With | P18494; P36003 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10604478}. |
| Modified Residue | MOD_RES 2; /note=N-acetylmethionine; /evidence=ECO:0007744|PubMed:22814378 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (8) |
| Cross Reference PDB | 1G6W; 1G6Y; 1HQO; 1JZR; 1K0A; 1K0B; 1K0C; 1K0D; |
| Mapped Pubmed ID | 10048020; 10388576; 10511541; 10574755; 10585968; 10611304; 10617575; 10640599; 10648962; 10648964; 10675903; 10676809; 10688190; 10748041; 10752606; 10801430; 10849793; 10861899; 10940235; 10940301; 10943358; 11096087; 11137008; 11223404; 11408486; 11447696; 11573346; 11687605; 11738587; 11751580; 11766884; 11779240; 11805837; 11832240; 11862619; 11931236; 11936870; 11948353; 11959975; 12039735; 12065404; 12093925; 12105276; 12149514; 12161628; 12165425; 12232791; 12489124; 12562760; 12606584; 12615539; 12620851; 12684011; 12702263; 12719473; 12777380; 12801414; 12890024; 12925776; 14517262; 14551258; 14560952; 14592448; 14610069; 14624857; 14625537; 14712063; 14755636; 15037545; 15098533; 15109261; 15133656; 15168540; 15189155; 15208260; 15282319; 15355224; 15456789; 15556413; 15621414; 15628874; 15806612; 15856224; 15866740; 15931169; 16096644; 16117685; 16123127; 16131495; 16263269; 16275904; 16293764; 16385730; 16405906; 16427819; 16554755; 16571726; 16675391; 16772049; 16793280; 16864574; 16879428; 16901730; 16954535; 17001037; 17041625; 17107617; 17133618; 17190613; 17234629; 17320905; 17324933; 17392510; 17482207; 17507672; 17514901; 17632572; 18243685; 18324784; 18332119; 18394190; 18400756; 18441120; 18460470; 18462383; 18467557; 18651095; 18660849; 18719252; 18798523; 18833194; 18845158; 18951365; 18955697; 19008939; 19015537; 19104072; 19124570; 19164897; 19164899; 19164904; 19164915; 19164928; 19239250; 19258323; 19275740; 19361397; 19383475; 19430540; 19521502; 19556854; 19712107; 19748512; 19752212; 19768774; 19774547; 19777209; 19841731; 19861427; 20071174; 20083897; 20206679; 20339590; 20356930; 20675578; 20726779; 20726897; 20740275; 20824085; 20868754; 21076138; 21078122; 21091436; 21183075; 21233211; 21339834; 21345375; 21354317; 21368275; 21374508; 21397710; 21402947; 21419850; 21439395; 21467567; 21497604; 21521246; 21658086; 21717344; 21730048; 21811098; 21832086; 21901113; 21902802; 21936815; 22052347; 22052348; 22052349; 22052351; 22052352; 22052353; 22094426; 22145302; 22156730; 22156731; 22169538; 22339593; 22363733; 22396539; 22419079; 22482457; 22511879; 22580344; 22617386; 22632966; 22684328; 22731490; 22879407; 22940803; 22949655; 22967940; 23077577; 23166488; 23167605; 23184930; 23294335; 23358190; 23379365; 23485338; 23498901; 23512891; 23605494; 23649250; 23733851; 23763759; 23764835; 23764836; 23797105; 23977222; 24040173; 24091626; 24185848; 24205798; 24236638; 24260565; 24366087; 24390581; 24408033; 24483210; 24738657; 24766537; 24938787; 24972093; 25131596; 25341659; 25373385; 25527290; 25620563; 25631286; 25654539; 25667942; 25977297; 26030507; 26031938; 26039159; 26110609; 26115123; 26333687; 26386407; 26419331; 26456335; 26473735; 26503767; 26702057; 26703187; 26713322; 26742083; 26760759; 26809710; 26908221; 26915272; 26996412; 27032776; 27183566; 27442849; 27481532; 27686217; 27693354; 27763256; 28007891; 2892826; 30231023; 32868289; 33857305; 34152451; 5573734; 7476941; 7592485; 7888172; 7891726; 7909168; 7953567; 8002570; 8066430; 8575202; 8978027; 8982451; 9356479; 9482721; 9611198; 9611819; 9758807; 9792709; 9820592; 9832522; 9872986; |
| Motif | |
| Gene Encoded By | |
| Mass | 40,271 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.4 mM for bis-(2-hydroxyethyl) disulfide (HEDS) {ECO:0000269|PubMed:19321443}; Note=kcat is 1.2 sec(-1) with bis-(2-hydroxyethyl) disulfide (HEDS) as substrate.; |
| Metal Binding | |
| Rhea ID | RHEA:16833 |
| Cross Reference Brenda |