| IED ID | IndEnz0002010075 |
| Enzyme Type ID | protease010075 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase UCHL3 EC 3.4.19.12 PfUCHL3 |
| Gene Name | UCHL3 PF3D7_1460400 |
| Organism | Plasmodium falciparum (isolate 3D7) |
| Taxonomic Lineage | cellular organisms Eukaryota Sar Alveolata Apicomplexa Aconoidasida Haemosporida (haemosporidians) Plasmodiidae Plasmodium Plasmodium (Laverania) Plasmodium falciparum Plasmodium falciparum (isolate 3D7) |
| Enzyme Sequence | MAKNDIWTPLESNPDSLYLYSCKLGQSKLKFVDIYGFNNDLLDMIPQPVQAVIFLYPVNDNIVSENNTNDKHNLKENFDNVWFIKQYIPNSCGTIALLHLYGNLRNKFELDKDSVLDDFFNKVNEMSAEKRGQELKNNKSIENLHHEFCGQVENRDDILDVDTHFIVFVQIEGKIIELDGRKDHPTVHCFTNGDNFLYDTGKIIQDKFIEKCKDDLRFSALAVIPNDNFDII |
| Enzyme Length | 232 |
| Uniprot Accession Number | Q8IKM8 |
| Absorption | |
| Active Site | ACT_SITE 92; /note=Nucleophile; /evidence=ECO:0000269|PubMed:20042598; ACT_SITE 164; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P09936 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:17371404, ECO:0000269|PubMed:19047059, ECO:0000269|PubMed:20042598, ECO:0000269|PubMed:31658303}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8 (PubMed:17371404, PubMed:31658303, PubMed:20042598). Essential for parasite blood stage survival (PubMed:20042598). {ECO:0000269|PubMed:17371404, ECO:0000269|PubMed:20042598, ECO:0000269|PubMed:31658303}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (7); Chain (1); Helix (9); Mutagenesis (3); Region (3); Site (4); Turn (3) |
| Keywords | 3D-structure;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 2WDT; 2WE6; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 26,899 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=301 nM for ubiquitin-AMC {ECO:0000269|PubMed:20042598}; Note=kcat is 3.2 sec(-1) with ubiquitin-AMC as substrate. {ECO:0000269|PubMed:20042598}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |