| IED ID | IndEnz0002010134 |
| Enzyme Type ID | protease010134 |
| Protein Name |
Signal peptide, CUB and EGF-like domain-containing protein 2 Protein You |
| Gene Name | scube2 you |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MGAVWTVRLLCLFLLLLNTRQSAALPHNTDQCAEGSDACHIDAICQNTPTSYKCTCKTGFKGDGKHCEDIDECDVEYNGGCVHECNNIPGNYRCTCLDGFHLAHDGHNCLDVDECVFNNGGCQHVCVNTMGSYECRCKQGFFLSDNQHTCIHRSVEGLSCMNKEHGCGHICKESPKGGVACECRPGFELAKNQRGCILTCNHGNGGCQHICEDTEQGPICRCHVRYMLHADGRTCVERDEMAPTAPDHNATSLAEVDKRVKRRLLMETCAVNNGGCDSTCKDTSTGVRCSCPVGFTLQPDGKSCKDIDECELHNGGCDHYCRNTIGSFECSCRKGFKLLTDERSCQDIDECFFERTCDHTCVNSPGSFQCVCNKGYTLYGLAHCGDINECSFNNGGCEHTCENTMGSFGCHCRAGYKLHWNKKDCIEAEDSPLVPPPARPTLNCNKQGGGELCYLTCQSQVHISSGAEDSYTVTCGMPLPCHTGGQWNGSYCPGSGIKTIATFKSGQGKCNLKRSHENLAHSFKTALSDKRATENVQFSFVSLHCASSSRQQRSRHGRKAGEEEGSFITAQFELDVNLEEVTAEGCDLTCVRRRSEKRLRKTIRTLRKSINREQFHLHFAGSEYELAKKLVRPADTPDHCGTGQILLDKKCVKCSVGTYYDGEQGRCFLCPPGTYQDEEGQVSCDVCPGPEGRGIPETAGARNISECAGQCRPGQFSHDGFVPCLPCPQGTYQPEVGRTSCFTCGGSLTTKYDGSVSFQNCETKVQCSPGHYYNTSTHRCIRCPVGTYQMEFGQNYCIACPGNTTTDFDGSTNIMQCKNRHCGGELGEFTGYIESPNYPGNYPANIECTWTITPPPKRRILVVVPEIYLPIEDECGDYLVMRKSSLPNSVTTYETCQTYERPIAFTSRSKKLWIQFRSNEGNSGKGFQVPYVTYDEDYQELIEDIVRDGRLYASENHQEILKDKKLMRALFDVLAHPQNFFNYTAQESREMFPKSFIRFLRSKVLRFLRP |
| Enzyme Length | 1010 |
| Uniprot Accession Number | Q5G872 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Lipid-binding protein required for SHH long-range signaling by binding to the dually lipid-modified SHH (ShhNp) and by promoting ShhNp mobilization, solubilization and release from the cell membrane. Acts by enhancing the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface. Synergizes with DISP1 to cause an increase in SHH secretion. Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis. {ECO:0000250|UniProtKB:Q9NQ36}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (19); Domain (10); Glycosylation (6); Region (1); Sequence conflict (8); Signal peptide (1) |
| Keywords | Calcium;Developmental protein;Disulfide bond;EGF-like domain;Glycoprotein;Reference proteome;Repeat;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9NQ36}. Cell surface {ECO:0000250|UniProtKB:Q9NQ36}. Note=Secreted and tethered at the cell surface. {ECO:0000250|UniProtKB:Q9NQ36}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. {ECO:0000250|UniProtKB:Q9NQ36}. |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10642786; 22609552; 24131633; 9007237; 9007249; |
| Motif | |
| Gene Encoded By | |
| Mass | 112,176 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |