| IED ID | IndEnz0002010166 |
| Enzyme Type ID | protease010166 |
| Protein Name |
Extracellular minor metalloprotease EC 3.4.24.- |
| Gene Name | smp |
| Organism | Serratia marcescens (strain ATCC 21074 / E-15) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Yersiniaceae Serratia unclassified Serratia Serratia marcescens (strain ATCC 21074 / E-15) |
| Enzyme Sequence | MPAQRMRSVIPPYMLRALLTRYAPQRDCALHTLNHVQSLLGNKPLRSPTEKNARAGERSAISTTPERHPTARQTGAQGGAAQQPRRAVDEAYDHLGVTYDFFWQAYRRNSVDNKGLPLVQRALRQGLPEQLSGTASRWCSETATARSSTVSPSPSTLVGHELTHGSDRERSRLIYYQQSGALNESLSDVFGSLVKQFHLQQTADKADWLIGAGLLAKGIKGKGLRSMSAPGTAYDDPLLGKDPQPASMKDYIQTKEDNGGVHLNSGIPNRAFYLAATVLGGFAGKKPVTSGMTRCATKRCRKTPTSDHLRPRHGETRAGLRTKRGDKVQQAWASGWQWSNETAADAQSGYGH |
| Enzyme Length | 352 |
| Uniprot Accession Number | Q06517 |
| Absorption | |
| Active Site | ACT_SITE 161; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; ACT_SITE 262; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius.; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (3); Metal binding (3); Propeptide (1); Region (3); Signal peptide (1) |
| Keywords | Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..? |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 38,515 |
| Kinetics | |
| Metal Binding | METAL 160; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 164; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 184; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |