| IED ID |
IndEnz0002010215 |
| Enzyme Type ID |
protease010215 |
| Protein Name |
Serine protease inhibitor
|
| Gene Name |
|
| Organism |
Lentinula edodes (Shiitake mushroom) (Lentinus edodes) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Basidiomycota
Agaricomycotina
Agaricomycetes
Agaricomycetidae
Agaricales
Omphalotaceae
Lentinula
Lentinula edodes (Shiitake mushroom) (Lentinus edodes)
|
| Enzyme Sequence |
SLETGRYLIHNGNNIVSRNLAEDRSLNPKRIVLLEPTDKIQLTWIIEKSGDEYILNNRGAPTAHIEDHVFALLIHQEGATKWSIEAVPRHGRNAYIIKGSDGKGWVAPDKAGEQIIYRTLIVGPSEPPTFPLNQVFQIIKLE |
| Enzyme Length |
142 |
| Uniprot Accession Number |
P81639 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Serine protease inhibitor. Active against beta-trypsin and alpha-chymotrypsin with dissociation constants of 0.35 nM and 40 nM respectively. Inhibits factor XIa, but not other enzymes involved in coagulation and fibrinolysis. Does not inhibit subtilisin, lysyl endopeptidase, arginyl endopeptidase or papain. {ECO:0000269|PubMed:10411656}. |
| Temperature Dependency |
|
| PH Dependency |
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Inactive at pH 2.0, activity is restored after 10 minutes incubation at pH 8.0. {ECO:0000269|PubMed:10411656}; |
| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Modified residue (1) |
| Keywords |
Acetylation;Direct protein sequencing;Protease inhibitor;Serine protease inhibitor |
| Interact With |
|
| Induction |
|
| Subcellular Location |
|
| Modified Residue |
MOD_RES 1; /note=N-acetylserine; /evidence=ECO:0000269|PubMed:10411656 |
| Post Translational Modification |
|
| Signal Peptide |
|
| Structure 3D |
|
| Cross Reference PDB |
- |
| Mapped Pubmed ID |
- |
| Motif |
|
| Gene Encoded By |
|
| Mass |
15,957 |
| Kinetics |
|
| Metal Binding |
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| Rhea ID |
|
| Cross Reference Brenda |
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