| IED ID | IndEnz0002010291 |
| Enzyme Type ID | protease010291 |
| Protein Name |
Thrombin-like enzyme BpSP-1 SVTLE BpSP-1 EC 3.4.21.- Fibrinogen-clotting enzyme Snake venom serine protease SVSP Thrombin-like enzyme BpSP-I SVTLE BpSP-I Fragment |
| Gene Name | |
| Organism | Bothrops pauloensis (Neuwied's lancehead) (Bothrops neuwiedi pauloensis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Bothrops Bothrops neuwiedi group Bothrops pauloensis (Neuwied's lancehead) (Bothrops neuwiedi pauloensis) |
| Enzyme Sequence | VIGGDECDINEHPFL |
| Enzyme Length | 15 |
| Uniprot Accession Number | P0DJF1 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited PMSF, benzamidine, leupeptin, and Cu(2+). {ECO:0000269|PubMed:19539638}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Thrombin-like enzyme that has high clotting activity upon bovine and human plasma. Has also fibrinogenoltic activity by rapidly hydrolyzing Aalpha chain of fibrinogen (FGA), and partially consumes Bbeta chain (FGB). Has high catalytic activity upon substrates such as TAME, and specific substrates for thrombin S-2238 and S-2288. Also hydrolyzes specific substrates for kallikrein (S-2266 and S-2302), however, no release of kinin upon plasma has been determined. When administered intraperitoneally in mice, causes defibrinogenation, making the plasma incoagulable. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (1); Domain (1); Non-terminal residue (1) |
| Keywords | Blood coagulation cascade activating toxin;Direct protein sequencing;Disulfide bond;Glycoprotein;Hemostasis impairing toxin;Hydrolase;Protease;Secreted;Serine protease;Sialic acid;Toxin |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. Contains sialic acid residues. {ECO:0000269|PubMed:19539638}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,658 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |