| IED ID | IndEnz0002010310 |
| Enzyme Type ID | protease010310 |
| Protein Name |
Probable glutamate carboxypeptidase VP8 EC 3.4.17.21 Protein VIVIPAROUS8 Protein WIDOW'S PEAK 1 |
| Gene Name | VP8 WPK1 Zm00001d034383 |
| Organism | Zea mays (Maize) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae PACMAD clade Panicoideae Andropogonodae Andropogoneae Tripsacinae Zea Zea mays (Maize) |
| Enzyme Sequence | MPHSVLARLPPGSVRLVAAFGLLLLVSLLVLHRRPGRPHVAAAAASDRLTDPSRSRLFLSQSPGANASIAADLRALTAGPHLAGTPASAGAAAHVLARLRAAGLQTLTREYEPLLSYPGHASLALLRPDGSLLARLSLEEPADEGRRVVPPYHAYAPSGGAVAEAVFVNLGREEDYVVLERLGVGVRGRVAVARRGGGYRGGVVARAADKGAVAVLIAGNADGGVERGVVLLGGPGDPLTPGWAATSGAERLKFDDKAVKQRFPSIPSMPVSAKTAAAIIRSLGGPAIPAEWKDGLGVDTGGLGPGPTLVNFTYQEDRKFYKIRDIFGIIKGQEEPDRYVILGNHRDAWTYGAVDPNSGTAALLDIARRLGIMLQSGWKPRRSIILCSWDGEEFGMIGSTEWVEDNLEDLHSKAVAYLNVDCAVQGVGFFAGSTPQLDKLLVDITRQVRDPDVTGKMVHDTWNEMSGGIKIERLARTDSDFAPFLHHAGIPSVDLYYGEEFPGYHTALDTYNWMEKHGDPFFLRHLAITEIWGLLALRLANDPVLPFDYQAYTSQLQEHIKTLSALTSNGHAVNLMNGCVNDLSGAAMEVLKEMKKLQQMDLYDEHARMRRRLLNDRLLLAERSFLQPEGLQGRGWFKHLLYSPPEDYESKLSFFPGIADAISRSANLSDKEQEVAMQHEVWKVCRAIQRAASVLRGEFSEQKPTNFSSLVTP |
| Enzyme Length | 713 |
| Uniprot Accession Number | A0A1D6L709 |
| Absorption | |
| Active Site | ACT_SITE 392; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q04609 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.; EC=3.4.17.21; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 3.4.17.21 |
| Enzyme Function | FUNCTION: Involved in the regulation of meristem development and seed maturation processes. Mediates regulation of embryonic regulatory genes and genes controlling abscisic acid (ABA) biosynthesis and turnover in developing seeds. May be required for the synthesis of small signaling molecules that integrates meristem and embryo formation in seeds. {ECO:0000269|PubMed:18203869}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Glycosylation (4); Metal binding (6); Region (1); Sequence conflict (3); Topological domain (2); Transmembrane (1) |
| Keywords | Cell membrane;Developmental protein;Glycoprotein;Growth regulation;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Single-pass type II membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 77,451 |
| Kinetics | |
| Metal Binding | METAL 345; /note=Zinc 1; via tele nitrogen; catalytic; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 355; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 355; /note=Zinc 2; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 393; /note=Zinc 2; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 421; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 505; /note=Zinc 2; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:Q04609 |
| Rhea ID | |
| Cross Reference Brenda |