| IED ID | IndEnz0002010319 |
| Enzyme Type ID | protease010319 |
| Protein Name |
Zinc metalloproteinase/disintegrin Cleaved into: Snake venom metalloproteinase brevilysin L4 SVMP Snake venom metalloproteinase hxl-1 EC 3.4.24.- ; Disintegrin salmosin-1 Platelet aggregation inhibitor ; Disintegrin salmosin-1 minor component Disintegrin brevicaudin-2b Fragment |
| Gene Name | |
| Organism | Gloydius brevicaudus (Korean slamosa snake) (Agkistrodon halys brevicaudus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Gloydius Gloydius brevicaudus (Korean slamosa snake) (Agkistrodon halys brevicaudus) |
| Enzyme Sequence | EAPKMCGVTQNWESYEPIKKASQSNLTPAHQRYIELVIVADHGMFTKYNGDSDKIREWVRQMVNTVDEIYSYMYIDVALAGLEIWSNEDLINVQPAAPHTLDSFGKWRERDLLHRIHHDNAMLLTAIDFDGPTIGLAYVGTMCKPKGSTGVVQDHSTINLRVAVTMAHEIGHNLGIHHDTGSCSCGGYSCIMSPVISHEPSKYFSDCSYTQCWDFIMNQKPQCILNKPLRTDTVSTPVSGNELLEAGEECDCGSPGNPCCDAATCKLRQGAQCAEGLCCDQCRFMKEGTICRRARGDDLDDYCNGISAGCPRNPFHA |
| Enzyme Length | 317 |
| Uniprot Accession Number | Q90WC0 |
| Absorption | |
| Active Site | ACT_SITE 169; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: [Snake venom metalloproteinase brevilysin L4]: metalloproteinase that impairs hemostasis in the envenomed animal. {ECO:0000250, ECO:0000269|PubMed:10446992, ECO:0000269|PubMed:10944460, ECO:0000269|PubMed:12615062, ECO:0000269|PubMed:14977354, ECO:0000269|PubMed:9722022, ECO:0000269|PubMed:9856345, ECO:0000269|Ref.3}.; FUNCTION: [Disintegrin salmosin-1]: Inhibits GPIIb/GPIIIa (ITGA2B/ITGB3) binding to immobilized fibrinogen with an IC(50) of 2.2 nM and ADP-induced platelet aggregation with an IC(50) of 131 nM, respectively. Inhibits angiogenesis. By binding to vitronectin receptor (alpha-V/beta-3 (ITGAV/ITGB3)), also induces apoptosis of endothelial cells by blocking their attachment to extracellular matrix proteins.; FUNCTION: [Disintegrin salmosin-1 minor component]: Inhibits platelet aggregation induced by ADP (IC(50) is 30 nM), collagen (IC(50) is 500 nM), thrombin and epinephrin (IC(50) is 160 nM). |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (3); Chain (3); Disulfide bond (9); Domain (2); Metal binding (7); Motif (1); Non-terminal residue (1); Propeptide (2) |
| Keywords | 3D-structure;Calcium;Cell adhesion impairing toxin;Direct protein sequencing;Disulfide bond;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Platelet aggregation inhibiting toxin;Protease;Secreted;Toxin;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (1) |
| Cross Reference PDB | 1L3X; |
| Mapped Pubmed ID | - |
| Motif | MOTIF 295..297; /note=Cell attachment site |
| Gene Encoded By | |
| Mass | 35,110 |
| Kinetics | |
| Metal Binding | METAL 35; /note="Calcium"; /evidence="ECO:0000250"; METAL 119; /note="Calcium"; /evidence="ECO:0000250"; METAL 168; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 172; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095"; METAL 178; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276"; METAL 223; /note="Calcium; via carbonyl oxygen"; /evidence="ECO:0000250"; METAL 226; /note="Calcium"; /evidence="ECO:0000250" |
| Rhea ID | |
| Cross Reference Brenda |