| IED ID | IndEnz0002010331 |
| Enzyme Type ID | protease010331 |
| Protein Name |
D-alanyl-D-alanine dipeptidase D-Ala-D-Ala dipeptidase EC 3.4.13.22 |
| Gene Name | ddpX vanX slr1679 |
| Organism | Synechocystis sp. (strain PCC 6803 / Kazusa) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Cyanobacteria/Melainabacteria group Cyanobacteria Synechococcales Merismopediaceae Synechocystis unclassified Synechocystis Synechocystis sp. PCC 6803 Synechocystis sp. (strain PCC 6803 / Kazusa) |
| Enzyme Sequence | MSFDTPLKPYLAIPIQDCGEPLAPINLEGVKSLKPHPYAQVGADYQGRSPYVLRTGVLKRLDQARLTLADIEPSWEILVFDAYRPIAVQQFMVDHTFAEIVARDGLQGQVLTPEQKENIYHQVYQIWAVPNNNPLTPPPHSTGAALDITLLDDLGQPVDMGGEIDELSARSLPNYYQTVEPNSDRQRKEFEQYQRRRELLNTIMESAGFLRHPGEWWHFSQGDQLWAWQYNQRHPDHQKIAYYGRVE |
| Enzyme Length | 247 |
| Uniprot Accession Number | P74268 |
| Absorption | |
| Active Site | ACT_SITE 215; /note=Proton donor/acceptor; /evidence=ECO:0000255|HAMAP-Rule:MF_01924 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=D-alanyl-D-alanine + H2O = 2 D-alanine; Xref=Rhea:RHEA:20661, ChEBI:CHEBI:15377, ChEBI:CHEBI:57416, ChEBI:CHEBI:57822; EC=3.4.13.22; Evidence={ECO:0000255|HAMAP-Rule:MF_01924, ECO:0000269|PubMed:9751644}; |
| DNA Binding | |
| EC Number | 3.4.13.22 |
| Enzyme Function | FUNCTION: Catalyzes hydrolysis of the D-alanyl-D-alanine dipeptide. May have a role in cell-wall turnover. {ECO:0000255|HAMAP-Rule:MF_01924, ECO:0000269|PubMed:9751644}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (3); Site (1) |
| Keywords | Cell wall biogenesis/degradation;Cytoplasm;Dipeptidase;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:9751644}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 28,437 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.6 mM for D-Ala-D-Ala {ECO:0000269|PubMed:9751644}; KM=8.3 mM for L-Ala-D-Ala {ECO:0000269|PubMed:9751644}; Note=kcat is 310 sec(-1) with D-Ala-D-Ala. kcat is 110 sec(-1) with L-Ala-D-Ala.; |
| Metal Binding | METAL 140; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_01924; METAL 147; /note=Zinc; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_01924; METAL 218; /note=Zinc; via pros nitrogen; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_01924 |
| Rhea ID | RHEA:20661 |
| Cross Reference Brenda |