| IED ID | IndEnz0002010343 |
| Enzyme Type ID | protease010343 |
| Protein Name |
Antihemorrhagic factor jMSF Japanese mamushi serum factor |
| Gene Name | |
| Organism | Gloydius blomhoffii (Mamushi) (Agkistrodon halys blomhoffi) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Gloydius Gloydius blomhoffii (Mamushi) (Agkistrodon halys blomhoffi) |
| Enzyme Sequence | MHFLVALVLLGQIIGSTLSSQVRGDLECDDREAKEWADQAVRYINEHKLHEYKQALNVIKNIVVVPWNGDLVAVFLKLNLLETECHVLDPTPVEKCTIRPQQNHAVEMDCDAKIMFDVETFKQDVFVKCHSTPDSVEDVRRNCPKCPILLSPRDPHVVDSVEYVLNKHNEQLSGHVYEVLEISRGQHKYEPEAFYVEFAIVEVNCTAQEAHDDHHHCHPNTAGEDHIAFCKATVFRSHASLEKPKHENFESDCVILDVKEGHAHSHLIEHHIGKYSTSPGQNSTVECVAECPVAFVNKEVPTDISDRHTTPVKGCPGKILHFQL |
| Enzyme Length | 324 |
| Uniprot Accession Number | Q5KQS1 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Suppress hemorrhage induced by metalloproteinases from the same venom (brevilysin-H3, -H4, -H6) and from habu venom (weak inhibition of the metalloproteinases HR2A). The non-hemorrhagic brevilysin-H2 is strongly inhibited by jMSF, whereas the brevilysin-L6 is not inhibited. Does not inhibit serine and cysteine proteases such as trypsin, chymotrypsin, thermolysin, and papain. The inhibition may occur by formation of a non-covalent complex between this protein and the proteinases at their metalloproteinase domains. {ECO:0000269|PubMed:18037153}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (7); Domain (2); Glycosylation (2); Motif (1); Signal peptide (1); Site (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Glycoprotein;Metalloenzyme inhibitor;Metalloprotease inhibitor;Protease inhibitor;Repeat;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000269|PubMed:18037153 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 23..25; /note=Cell attachment site; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 36,663 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |