| IED ID | IndEnz0002010381 |
| Enzyme Type ID | protease010381 |
| Protein Name |
Lysosomal acid phosphatase LAP EC 3.1.3.2 |
| Gene Name | acp2 |
| Organism | Xenopus laevis (African clawed frog) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Xenopus Xenopus laevis (African clawed frog) |
| Enzyme Sequence | MADGSCLGSGPQLGLIALLVVLLFSAVPLAQSRELRFVTLVYRHGDRSPVHGYPTDVHKESVWPQGYGQLTQVGMKQHWDLGQELRARYKGFLNESYNRHEIYVRSTDVDRTLMSAEANLAGLYPPEGPQIFNPNITWQPIPIHTIPESEDQLLKFPISPCPAYVKLQEETRQSAEYINMTTTYKAFLQMVANKTGLSDCTLESVWSVYDTLFCEKTHNFSLPTWATADVLSKLNKLKDFSFVFLFGVHERVKKARLQGGVLVDQILKNMTAAANNASNGLKLLAYSAHDSTLGALQLALDVYNGKQAPYASCHIFELYKEDSGNFTVQMYFRNESGKTPYPVSLPGCAHACPLQDFQSLLQPILAQDWEEECQTTSFIMTEETIIGLTIGAIALFIIIVVLMLLSCNEPKDDGYQHVSDEGDDHETKGLAM |
| Enzyme Length | 432 |
| Uniprot Accession Number | B1H1P9 |
| Absorption | |
| Active Site | ACT_SITE 44; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 290; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a phosphate monoester + H2O = an alcohol + phosphate; Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879, ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2; |
| DNA Binding | |
| EC Number | 3.1.3.2 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (3); Glycosylation (7); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Membrane;Signal;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:P11117}; Single-pass membrane protein {ECO:0000255}; Lumenal side {ECO:0000250|UniProtKB:P11117}. Lysosome lumen {ECO:0000250|UniProtKB:P11117}. Note=The soluble form arises by proteolytic processing of the membrane-bound form. {ECO:0000250|UniProtKB:P11117}. |
| Modified Residue | |
| Post Translational Modification | PTM: The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen (By similarity). {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..32; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 48,243 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15017 |
| Cross Reference Brenda |