| IED ID | IndEnz0002010386 |
| Enzyme Type ID | protease010386 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 3 EC 3.4.19.12 Deubiquitinating enzyme 3 Ubiquitin thioesterase 3 Ubiquitin-specific-processing protease 3 |
| Gene Name | UBP3 YER151C |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MNMQDANKEESYSMYPKTSSPPPPTPTNMQIPIYQAPLQMYGYTQAPYLYPTQIPAYSFNMVNQNQPIYHQSGSPHHLPPQNNINGGSTTNNNNINKKKWHSNGITNNNGSSGNQGANSSGSGMSYNKSHTYHHNYSNNHIPMMASPNSGSNAGMKKQTNSSNGNGSSATSPSYSSYNSSSQYDLYKFDVTKLKNLKENSSNLIQLPLFINTTEAEFAAASVQRYELNMKALNLNSESLENSSVEKSSAHHHTKSHSIPKHNEEVKTETHGEEEDAHDKKPHASKDAHELKKKTEVKKEDAKQDRNEKVIQEPQATVLPVVDKKEPEESVEENTSKTSSPSPSPPAAKSWSAIASDAIKSRQASNKTVSGSMVTKTPISGTTAGVSSTNMAAATIGKSSSPLLSKQPQKKDKKYVPPSTKGIEPLGSIALRMCFDPDFISYVLRNKDVENKIPVHSIIPRGIINRANICFMSSVLQVLLYCKPFIDVINVLSTRNTNSRVGTSSCKLLDACLTMYKQFDKETYEKKFLENADDAEKTTESDAKKSSKSKSFQHCATADAVKPDEFYKTLSTIPKFKDLQWGHQEDAEEFLTHLLDQLHEELISAIDGLTDNEIQNMLQSINDEQLKVFFIRNLSRYGKAEFIKNASPRLKELIEKYGVINDDSTEENGWHEVSGSSKRGKKTKTAAKRTVEIVPSPISKLFGGQFRSVLDIPNNKESQSITLDPFQTIQLDISDAGVNDLETAFKKFSEYELLPFKSSSGNDVEAKKQTFIDKLPQVLLIQFKRFSFINNVNKDNAMTNYNAYNGRIEKIRKKIKYGHELIIPEESMSSITLKNNTSGIDDRRYKLTGVIYHHGVSSDGGHYTADVYHSEHNKWYRIDDVNITELEDDDVLKGGEEASDSRTAYILMYQKRN |
| Enzyme Length | 912 |
| Uniprot Accession Number | Q01477 |
| Absorption | |
| Active Site | ACT_SITE 469; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 861; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:12778054, ECO:0000269|PubMed:17632125}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Has an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety in natural or engineered linear fusion proteins, irrespective of their size or the presence of an N-terminal extension to ubiquitin (PubMed:12778054, PubMed:17632125). Plays a role in regulation of silencing by interacting with SIR4 (PubMed:8752220). Also, in conjunction with BRE5, cleaves ubiquitin, leading to the subsequent mono-ubiquitination of SEC23 (PubMed:12778054). Required for ribophagy, a process which relocalizes ribosomal particles into the vacuole for degradation in response to starvation (PubMed:20508643). {ECO:0000269|PubMed:12778054, ECO:0000269|PubMed:17632125, ECO:0000269|PubMed:20508643, ECO:0000269|PubMed:8752220}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (1); Chain (1); Compositional bias (4); Domain (1); Helix (1); Region (4) |
| Keywords | 3D-structure;Hydrolase;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | P53741; P25694; P36037; P32485 |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 2QIY; |
| Mapped Pubmed ID | 10527495; 10570463; 11076031; 11283351; 11722014; 11864977; 12454057; 14593109; 14690591; 15299026; 15449308; 15635103; 15699485; 15955808; 16027361; 16186126; 16429126; 16554755; 16997324; 1710619; 17158346; 17556048; 17986446; 18391941; 18394190; 18454128; 18498751; 18508269; 18719252; 19182805; 19264099; 19447341; 19489724; 19536198; 19734957; 19841731; 19920177; 20074044; 20738257; 20844764; 20846524; 20876584; 21151954; 21179020; 21317551; 21548784; 21743437; 22150273; 22277779; 22718752; 22842922; 22908043; 23202731; 23208446; 23222640; 23476013; 23545414; 23645675; 23733851; 24040173; 24307682; 24596250; 24616224; 24760971; 24862571; 25092318; 25468960; 25473596; 25704822; 25795416; 26192197; 26422135; 26503604; 26503781; 26586132; 26646153; 26657849; 26670610; 26787877; 26837754; 26852120; 27145142; 27308583; 27442630; 27693354; 27698423; 27715443; 27807036; 27821475; 29027900; 29924334; 33524398; 8643628; 9644204; |
| Motif | |
| Gene Encoded By | |
| Mass | 101,917 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |