| IED ID | IndEnz0002010388 |
| Enzyme Type ID | protease010388 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 36 EC 3.4.19.12 Deubiquitinating enzyme 36 Protein scrawny Ubiquitin thioesterase 36 Ubiquitin-specific-processing protease 36 |
| Gene Name | Usp36 scny GE21508 |
| Organism | Drosophila yakuba (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila yakuba (Fruit fly) |
| Enzyme Sequence | MPVSMAVCETANVVNAALRESLGGNSSAAGSSIDQAKSGEDSNGSLQNHIVANAKRILMAKIEYEEVPNYHESVLENLKSKYIVIKPGNPGAINGFGGKNNTGKVVGANGHDNNGARKQAEHPNNQSHHNNHNNHPHPTSNPNELPKPKRVLYPRENIRIGWKQSERKWQVGTGMINVGNTCYLNSTLQALLHIPALANWLVSEQAHLENCNVAESGGGCIVCAMAKTLLATQSNQSAVRPFLIYSKLKQICKHMVVGRQEDAHEFLRFLVEAMERAYLMRFRNYKELDQLVKETTPLGQIFGGYLRSEVRCLSCNHVSITFQHFQDLLLDIRKADSLEDAFEGHFSRERLEDMGYKCEGCKKKVSATKQFSLERAPITLCIQLKRFSMIGNKLTKQISLKPRIDLSKYAARSPAAQAQPLTYRLVSMVTHLGVSQHCGHYTAIGSTDTGSYYNFDDSYVRPIAMQSVCNTNAYIMFYELDLSQAASPAANRPNGVRLTNGHSTTPVPAATVSSPSPTRFIGPQLPPGGVNGYSNGNAQKTAIQFKQHHQQSQQNGFQLGTGKFQDTAKPPLVGAHAKGDANPVPTANGNKSSSTSSNSSSNHKSINQQQYLPISSEDEDSEDEMTPRPTTAQLPSMPKMTDDHTEKPKSPVKIQVKTPVKTPLKSLVPYESASEEEEAPQPNPRKRRSEEDSSESDQESGQTNGHSKTNGSLTNGSASSSVHVNNSKQKTDAIDEIFKSLKKSADSDDDDDEEESSIQLTNGWHPQKQSQSQSKAPPSPKTPPSPAVIKSKTGIWKVTRNDEVDDIDDDDDEEDEAPAKIQTPSKTHRNPFSSTKPSTESPATPGAKRQKLLNGSAVKSHQQPRVGNGYQSEATSNGSTINELLKQSHRGYGSSVLSWNGKPAELEKEPFELVCAKRIAGHGSVDGSDIVESSVAVNASSGSDSNDVVVIALLVDAREQRQRDLDDDEENEMDRGRQRKVKSSGSAKANNASNSTPGYNPFQEYEGQKRWNKNGGGGGGFPRFYNQNFRQNFQQRNKFKFNRFGGPGSAKFQQQRALQRHLAAGGGFSRRQPSAQQQQQQS |
| Enzyme Length | 1082 |
| Uniprot Accession Number | B4PIW8 |
| Absorption | |
| Active Site | ACT_SITE 182; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 440; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Controls selective autophagy activation by ubiquitinated proteins. {ECO:0000250|UniProtKB:Q9VRP5}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (12); Domain (1); Modified residue (13); Region (5) |
| Keywords | Hydrolase;Nucleus;Phosphoprotein;Protease;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}. |
| Modified Residue | MOD_RES 514; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 516; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 658; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 662; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 672; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 674; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 747; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 779; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 782; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 785; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 823; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 841; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 844; /note=Phosphothreonine; /evidence=ECO:0000250 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 118,583 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |