| IED ID | IndEnz0002010394 |
| Enzyme Type ID | protease010394 |
| Protein Name |
Serralysin B EC 3.4.24.40 Secreted protease B ProB |
| Gene Name | prtB |
| Organism | Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Pectobacteriaceae Dickeya Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) |
| Enzyme Sequence | MQQNEKASLNTSAAAAKAGSYTGYADVYDFWYYHQRGDGLTVNGKPSYTTDKAVSEGLTRPHTTWNGDNVFGKAANLTYSFLNTFSSTPNGHTGPVKFTPVQMQQAKLSLQSWADAANLTFTEVSPNQKANITFANYTRNADGSLNTDTQAYAAYPGTHPVSGSAWFNYNQSSIRNPDTDEYGRHSFTHEIGHALGLSHPAEYNAGEGDISYKNSAAYAEDSRQFSIMSYWEVENTGGDFKGHYSAGPLMDDIAAIQKLYGANMTTRTGDTVYGFNSNTDRDFYTATNSSKALVFSVWDAGGTDTFDFSGYSNNQRINLNEGSFSDVGGLKGNVSIAHGVTIENAIGGSGNDILIGNGADNILQGGAGDDVLYGSTGADTLTGGAGRDIFVYGSGQDSTVSAYDWITDFQTGIDKIDLSAFRNEGQLSFVQDQFTGKGQEVMLQWDAANSTTNLWLHEAGHSSVDFLVRIVGQTAQSDIIV |
| Enzyme Length | 481 |
| Uniprot Accession Number | P16316 |
| Absorption | |
| Active Site | ACT_SITE 190; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage of bonds with hydrophobic residues in P1'.; EC=3.4.24.40; |
| DNA Binding | |
| EC Number | 3.4.24.40 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Metal binding (38); Propeptide (1); Repeat (3) |
| Keywords | Calcium;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Secreted;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 51,770 |
| Kinetics | |
| Metal Binding | METAL 189; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 193; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 230; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 267; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 269; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 271; /note=Calcium 1; /evidence=ECO:0000250; METAL 299; /note=Calcium 1; /evidence=ECO:0000250; METAL 301; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 302; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250; METAL 304; /note=Calcium 1; /evidence=ECO:0000250; METAL 304; /note=Calcium 2; /evidence=ECO:0000250; METAL 341; /note=Calcium 2; via carbonyl oxygen; /evidence=ECO:0000250; METAL 343; /note=Calcium 2; /evidence=ECO:0000250; METAL 348; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 350; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 352; /note=Calcium 3; /evidence=ECO:0000250; METAL 357; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 359; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 361; /note=Calcium 4; /evidence=ECO:0000250; METAL 365; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 366; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250; METAL 367; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 368; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250; METAL 370; /note=Calcium 3; /evidence=ECO:0000250; METAL 370; /note=Calcium 5; /evidence=ECO:0000250; METAL 374; /note=Calcium 4; via carbonyl oxygen; /evidence=ECO:0000250; METAL 377; /note=Calcium 6; via carbonyl oxygen; /evidence=ECO:0000250; METAL 379; /note=Calcium 4; /evidence=ECO:0000250; METAL 379; /note=Calcium 6; /evidence=ECO:0000250; METAL 383; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250; METAL 384; /note=Calcium 7; via carbonyl oxygen; /evidence=ECO:0000250; METAL 385; /note=Calcium 5; via carbonyl oxygen; /evidence=ECO:0000250; METAL 386; /note=Calcium 7; via carbonyl oxygen; /evidence=ECO:0000250; METAL 388; /note=Calcium 5; /evidence=ECO:0000250; METAL 388; /note=Calcium 7; /evidence=ECO:0000250; METAL 397; /note=Calcium 6; /evidence=ECO:0000250; METAL 404; /note=Calcium 6; /evidence=ECO:0000250; METAL 414; /note=Calcium 7; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |