| IED ID | IndEnz0002010461 |
| Enzyme Type ID | protease010461 |
| Protein Name |
Lon protease homolog 2, peroxisomal EC 3.4.21.53 |
| Gene Name | LON1 OsI_32159 |
| Organism | Oryza sativa subsp. indica (Rice) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Oryzoideae Oryzeae Oryzinae Oryza Oryza sativa (Rice) Oryza sativa subsp. indica (Rice) |
| Enzyme Sequence | MADAAVELPGRLAILPFRNKVLLPGAIVRIRCTNPSSVKLVEQELWQREEKGLIGVLPVHDSEAAGSLLSPGVGSDSGEGGSKAPGGSAGESTKQDTKNGKETIHWHSRGVAARALHLSRGVEKPSGRVTYIVVLEGLCRFSVQELSARGSYHVARVSRLDMTKTELEHAEQDPDLIALSRQFKATAMELISVLEQKQKTVGRTKVLLETVPVYRLADIFVASFEISFEEQLSMLDSVDLKVRLSKATELVDRHLQSILVAEKITQKVEGQLSKSQKEFLLRQQMRAIKEELGDNDDDEDDVAALERKMQNAGMPANIWKHAQRELRRLRKMQPQQPGYSSSRTYLELLAELPWQKVSEERELDLRAAKESLDRDHYGLTKVKQRIIEYLAVRKLKPDARGPVLCFVGPPGVGKTSLASSIAKALNRKFIRISLGGVKDEADIRGHRRTYIGSMPGRLIDGLKRVSVSNPVMLLDEIDKTGSDVRGDPASALLEVLDPEQNKTFNDHYLNVPFDLSKVIFVATANRMQPIPPPLLDRMEVIELPGYTPEEKLKIAMKHLIPRVLEQHGLSSTYLQIPEAMVRLIIERYTREAGVRNLERNLAALARAAAVKVAEQDSVLRLGKEIQPITTTLLDSRLADGGEVEMEVIPMGQDISNTYENPSPMIVDEAMLEKVLGPPRFDDSEAADRVASPGVSVGLVWTSFGGEVQFVEATAMVGKGDLHLTGQLGDVIKESAQLALTWVRARAADLNLSPTSDINLLESRDIHIHFPAGAVPKDGPSAGVTLVTSLVSLFSHRKVRADTAMTGEMTLRGLVLPVGGVKDKVLAAHRYGIKRVILPERNMKDLAEVPAPILSGLEILLVKRIEEVLDHAFEGGCPLRPHSKL |
| Enzyme Length | 884 |
| Uniprot Accession Number | B8BDV1 |
| Absorption | |
| Active Site | ACT_SITE 780; /evidence=ECO:0000255|HAMAP-Rule:MF_03121; ACT_SITE 823; /evidence=ECO:0000255|HAMAP-Rule:MF_03121 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins in presence of ATP.; EC=3.4.21.53; Evidence={ECO:0000255|HAMAP-Rule:MF_03121}; |
| DNA Binding | |
| EC Number | 3.4.21.53 |
| Enzyme Function | FUNCTION: ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import. {ECO:0000255|HAMAP-Rule:MF_03121}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 408..415; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_03121 |
| Features | Active site (2); Chain (1); Domain (2); Motif (1); Nucleotide binding (1); Region (1); Sequence conflict (1) |
| Keywords | ATP-binding;Hydrolase;Nucleotide-binding;Peroxisome;Protease;Reference proteome;Serine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Peroxisome matrix {ECO:0000255|HAMAP-Rule:MF_03121}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 882..884; /note=Microbody targeting signal; /evidence=ECO:0000255|HAMAP-Rule:MF_03121 |
| Gene Encoded By | |
| Mass | 97,336 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |