| IED ID | IndEnz0002010463 |
| Enzyme Type ID | protease010463 |
| Protein Name |
Linocin-M18 EC 3.4.-.- |
| Gene Name | lin |
| Organism | Brevibacterium linens |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Micrococcales Brevibacteriaceae Brevibacterium Brevibacterium linens |
| Enzyme Sequence | MNNLYRELAPIPGPAWAEIEEEARRTFKRNIAGRRIVDVAGPTGFETSAVTTGHIRDVQSETSGLQVKQRIVQEYIELRTPFTVTRQAIDDVARGSGDSDWQPVKDAATTIAMAEDRAILHGLDAAGIGGIVPGSSNAAVAIPDAVEDFADAVAQALSVLRTVGVDGPYSLLLSSAEYTKVSESTDHGYPIREHLSRQLGAGEIIWAPALEGALLVSTRGGDYELHLGQDLSIGYYSHDSETVELYLQETFGFLALTDESSVPLSL |
| Enzyme Length | 266 |
| Uniprot Accession Number | Q45296 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: Bacteriocin that has antimicrobial activity against a wide range of Gram-positive bacteria, including L.monocytogenes, B.cereus and S.aureus. May have a protease activity. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermolabile. Inactivated after 5 min at 80 degrees Celsius. {ECO:0000269|PubMed:7986050}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5-9. {ECO:0000269|PubMed:7986050}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Sequence conflict (1) |
| Keywords | Antibiotic;Antimicrobial;Bacteriocin;Direct protein sequencing;Hydrolase;Protease;Secreted |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 28,597 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |