| IED ID | IndEnz0002010484 |
| Enzyme Type ID | protease010484 |
| Protein Name |
Neuroendocrine convertase 1 NEC 1 EC 3.4.21.93 Furin homolog PC3 Prohormone convertase 1 Propeptide-processing protease Proprotein convertase 1 PC1 |
| Gene Name | Pcsk1 Att-1 Nec-1 Nec1 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MEQRGWTLQCTAFAFFCVWCALNSVKAKRQFVNEWAAEIPGGQEAASAIAEELGYDLLGQIGSLENHYLFKHKSHPRRSRRSALHITKRLSDDDRVTWAEQQYEKERSKRSVQKDSALDLFNDPMWNQQWYLQDTRMTAALPKLDLHVIPVWEKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDLTNENKHGTRCAGEIAMQANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKTVEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIFALALEANPNLTWRDMQHLVVWTSEYDPLASNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRNVPEKKECVVKDNNFEPRALKANGEVIVEIPTRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAVGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPVGTWTLKITDMSGRMQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDRRGVEKMVNVVEKRPTQKSLNGNLLVPKNSSSSNVEGRRDEQVQGTPSKAMLRLLQSAFSKNALSKQSPKKSPSAKLSIPYESFYEALEKLNKPSKLEGSEDSLYSDYVDVFYNTKPYKHRDDRLLQALMDILNEEN |
| Enzyme Length | 753 |
| Uniprot Accession Number | P63239 |
| Absorption | |
| Active Site | ACT_SITE 167; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 208; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 382; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.; EC=3.4.21.93; |
| DNA Binding | |
| EC Number | 3.4.21.93 |
| Enzyme Function | FUNCTION: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP. {ECO:0000269|PubMed:16384863}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.5-6.5.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (7); Chain (1); Compositional bias (1); Disulfide bond (3); Domain (2); Glycosylation (2); Helix (4); Propeptide (1); Region (1); Sequence conflict (7); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Calcium;Cleavage on pair of basic residues;Cytoplasmic vesicle;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Serine protease;Signal;Zymogen |
| Interact With | Itself |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle. Note=Localized in the secretion granules. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000255 |
| Structure 3D | NMR spectroscopy (3) |
| Cross Reference PDB | 1KN6; 2KDT; 2KE3; |
| Mapped Pubmed ID | 10701998; 10894496; 10945231; 11076772; 12049785; 12136131; 12145326; 12358783; 12466851; 12584191; 12787078; 12933680; 12950171; 1354647; 1429684; 14614908; 14630721; 14681479; 14684599; 14693708; 15018810; 15056274; 15075450; 15587270; 15779921; 15956344; 16163737; 16407210; 16476726; 16602821; 16627579; 16644867; 16951258; 16987237; 17050541; 17339028; 17490633; 17537793; 17565604; 17645548; 1765368; 17698597; 17938243; 18096669; 18356286; 18410501; 18554181; 18554416; 18706454; 18723011; 18940205; 19156209; 19376969; 19628676; 19837164; 1988934; 20052009; 20138042; 20412386; 20413507; 20610561; 20657753; 20876279; 21677750; 21880149; 21888904; 21935423; 22396549; 22421509; 22579794; 22980982; 23027401; 23056534; 24101515; 24227653; 24828610; 24855103; 25153248; 25186293; 25784544; 26373413; 26778167; 26786350; 26901059; 27534441; 27626380; 27941249; 27998058; 28355570; 28476408; 28576773; 29198128; 29800682; 31504391; 31852976; 31974728; 32248978; 32694784; 7730317; 7805868; 7819324; 7925129; 8003017; 8011166; 8114702; 8198617; 8223273; 8262946; 8389457; 8397508; 8662221; 8698813; 8824815; 8891276; 8940009; 9242664; 9291583; 9584121; 9885250; |
| Motif | |
| Gene Encoded By | |
| Mass | 84,174 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.93; |