| IED ID | IndEnz0002010494 |
| Enzyme Type ID | protease010494 |
| Protein Name |
Intracisternal A-particle Pol-related polyprotein Cleaved into: Reverse transcriptase/ribonuclease H RT EC 2.7.7.49 EC 2.7.7.7 EC 3.1.26.4 ; Integrase IN Fragment |
| Gene Name | pol |
| Organism | Hamster intracisternal a-particle H18 (IAP-H18) |
| Taxonomic Lineage | Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae unclassified Retroviridae Intracisternal A-particles Hamster intracisternal a-particle H18 (IAP-H18) |
| Enzyme Sequence | LGAVEASRPIPWKTEEPLWVSQWPLSSEKLEAVTRLVQEQERLGHLEPSTSPWNTPIFVIKKKSGKWRLLHDLRAINNQMHLFGPVQRGLPLLSALPQDWKLIIIDIKDCFFSIPLYPRDRPRFAFTIPSLNHMEPDKRFQWKVLPQGMANSPTICQLYVQEALEPIRKQFTSLIVIHYMDDILICHKELDVLQKAFPMLVAELKQWGLEIASEKVQIADTGLFLGSKITPKNIVPQKIEIRKDHLQTLNDFQKLLGDINWLRPFLKIPSADLKPLFDLLEGEPHISSPRKFTPAAHRALQMVEEALQEAQITTNSPAKIIDWYPDAVAQPRSRIKAAVTHFGRDPDSLIVPYTAAQVQTLAATSSDWAVLVTSFSGQIDNHFPKHPILQFALNQAIVFPQVTAKDPLPDGTVVYTDGSKTGLGAYVVKDRVISKQYNETSPQVVECLIVLEVLEAFPGPLNIVSDSSYVVNAVNLLEIAGIIRSSSRVANIFQKIQAALLNRRFPVFITHVRAHSGLPGPMSLGNDLADKATKLVATALSTHAQAAKEFHKRFHVTAETLRRRFALSRKEAREIVTQCQNCCEFLPTPHMGINPRGIRPLQMWQMDVTHIPSFGRLQYVHVSVDTCSGVMFATPLTGEKASYVIQHCLEAWSAWGKPRIKTDNGPAYTSQKFRQFCRQMDVTHLTGLPYNPQGQGIVERAHRTLKSYLIKQKGSIEDVLPSVPRVAVSMALFTLNFLNCDAQGHTAADRHSLEPDRPKEMVKWKDVLTDLWKGPDPILIRSRGAVCVFPQEEENPLWIPERLTRRAPSQLQNKKDGGQVGDEDSPTRDDGSYDGRVTVGHHVYLPPTDASDAECPGVPIFHK |
| Enzyme Length | 863 |
| Uniprot Accession Number | P04026 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-ProRule:PRU00405}; CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|PROSITE-ProRule:PRU00405}; CATALYTIC ACTIVITY: Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.4; Evidence={ECO:0000255|PROSITE-ProRule:PRU00408}; |
| DNA Binding | DNA_BIND 760..809; /note=Integrase-type; /evidence=ECO:0000255|PROSITE-ProRule:PRU00506 |
| EC Number | 2.7.7.49; 2.7.7.7; 3.1.26.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Compositional bias (1); DNA binding (1); Domain (3); Metal binding (13); Non-terminal residue (1); Region (1); Site (1); Zinc finger (1) |
| Keywords | DNA integration;DNA recombination;DNA-binding;Endonuclease;Hydrolase;Magnesium;Metal-binding;Multifunctional enzyme;Nuclease;Nucleotidyltransferase;RNA-directed DNA polymerase;Transferase;Transposable element;Viral genome integration;Virus entry into host cell;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 97,037 |
| Kinetics | |
| Metal Binding | METAL 106; /note=Magnesium 1; catalytic; for reverse transcriptase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00405; METAL 181; /note=Magnesium; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00405; METAL 182; /note=Magnesium; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00405; METAL 417; /note=Magnesium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 446; /note=Magnesium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 466; /note=Magnesium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 530; /note=Magnesium; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 551; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450; METAL 555; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450; METAL 579; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450; METAL 582; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450; METAL 607; /note=Magnesium; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00457; METAL 663; /note=Magnesium; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU00457 |
| Rhea ID | RHEA:22508 |
| Cross Reference Brenda |