| IED ID | IndEnz0002010495 |
| Enzyme Type ID | protease010495 |
| Protein Name |
Pol polyprotein Cleaved into: Protease Retropepsin EC 3.4.23.- ; Reverse transcriptase/ribonuclease H RT EC 2.7.7.49 EC 3.1.26.13 Exoribonuclease H EC 3.1.13.2 ; Deoxyuridine 5'-triphosphate nucleotidohydrolase dUTPase EC 3.6.1.23 ; Integrase IN EC 2.7.7.- EC 3.1.-.- |
| Gene Name | pol |
| Organism | Feline immunodeficiency virus (isolate Petaluma) (FIV) |
| Taxonomic Lineage | Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae Orthoretrovirinae Lentivirus Feline immunodeficiency virus Feline immunodeficiency virus (isolate Petaluma) (FIV) |
| Enzyme Sequence | KEFGKLEGGASCSPSESNAASSNAICTSNGGETIGFVNYNKVGTTTTLEKRPEILIFVNGYPIKFLLDTGADITILNRRDFQVKNSIENGRQNMIGVGGGKRGTNYINVHLEIRDENYKTQCIFGNVCVLEDNSLIQPLLGRDNMIKFNIRLVMAQISDKIPVVKVKMKDPNKGPQIKQWPLTNEKIEALTEIVERLEKEGKVKRADSNNPWNTPVFAIKKKSGKWRMLIDFRELNKLTEKGAEVQLGLPHPAGLQIKKQVTVLDIGDAYFTIPLDPDYAPYTAFTLPRKNNAGPGRRFVWCSLPQGWILSPLIYQSTLDNIIQPFIRQNPQLDIYQYMDDIYIGSNLSKKEHKEKVEELRKLLLWWGFETPEDKLQEEPPYTWMGYELHPLTWTIQQKQLDIPEQPTLNELQKLAGKINWASQAIPDLSIKALTNMMRGNQNLNSTRQWTKEARLEVQKAKKAIEEQVQLGYYDPSKELYAKLSLVGPHQISYQVYQKDPEKILWYGKMSRQKKKAENTCDIALRACYKIREESIIRIGKEPRYEIPTSREAWESNLINSPYLKAPPPEVEYIHAALNIKRALSMIKDAPIPGAETWYIDGGRKLGKAAKAAYWTDTGKWRVMDLEGSNQKAEIQALLLALKAGSEEMNIITDSQYVINIILQQPDMMEGIWQEVLEELEKKTAIFIDWVPGHKGIPGNEEVDKLCQTMMIIEGDGILDKRSEDAGYDLLAAKEIHLLPGEVKVIPTGVKLMLPKGYWGLIIGKSSIGSKGLDVLGGVIDEGYRGEIGVIMINVSRKSITLMERQKIAQLIILPCKHEVLEQGKVVMDSERGDNGYGSTGVFSSWVDRIEEAEINHEKFHSDPQYLRTEFNLPKMVAEEIRRKCPVCRIIGEQVGGQLKIGPGIWQMDCTHFDGKIILVGIHVESGYIWAQIISQETADCTVKAVLQLLSAHNVTELQTDNGPNFKNQKMEGVLNYMGVKHKFGIPGNPQSQALVENVNHTLKVWIQKFLPETTSLDNALSLAVHSLNFKRRGRIGGMAPYELLAQQESLRIQDYFSAIPQKLQAQWIYYKDQKDKKWKGPMRVEYWGQGSVLLKDEEKGYFLIPRRHIRRVPEPCALPEGDE |
| Enzyme Length | 1124 |
| Uniprot Accession Number | P16088 |
| Absorption | |
| Active Site | ACT_SITE 68; /note=For protease activity |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.; EC=3.1.26.13; CATALYTIC ACTIVITY: Reaction=3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.; EC=3.1.13.2; CATALYTIC ACTIVITY: Reaction=dUTP + H2O = diphosphate + dUMP + H(+); Xref=Rhea:RHEA:10248, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:61555, ChEBI:CHEBI:246422; EC=3.6.1.23; CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-ProRule:PRU00405}; |
| DNA Binding | DNA_BIND 1067..1115; /note=Integrase-type; /evidence=ECO:0000255|PROSITE-ProRule:PRU00506 |
| EC Number | 3.4.23.-; 2.7.7.49; 3.1.26.13; 3.1.13.2; 3.6.1.23; 2.7.7.-; 3.1.-.- |
| Enzyme Function | FUNCTION: During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (29); Chain (4); DNA binding (1); Domain (4); Helix (9); Metal binding (9); Turn (6); Zinc finger (1) |
| Keywords | 3D-structure;Aspartyl protease;DNA integration;DNA recombination;DNA-binding;Endonuclease;Hydrolase;Metal-binding;Multifunctional enzyme;Nuclease;Nucleotide metabolism;Nucleotidyltransferase;Protease;RNA-directed DNA polymerase;Reference proteome;Transferase;Viral genome integration;Virus entry into host cell;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Cleavage sites that yield the mature proteins remain to be determined. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (20) |
| Cross Reference PDB | 1B11; 1DUT; 1F7D; 1F7K; 1F7N; 1F7O; 1F7P; 1F7Q; 1F7R; 1FIV; 2FIV; 2HAH; 3FIV; 3OGP; 3OGQ; 4FIV; 4MQ3; 4PA1; 5FIV; 6FIV; |
| Mapped Pubmed ID | 10651036; 10957629; 17212810; 21636894; 25199694; 9827997; |
| Motif | |
| Gene Encoded By | |
| Mass | 127,494 |
| Kinetics | |
| Metal Binding | METAL 601; /note=Magnesium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 601; /note=Magnesium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 634; /note=Magnesium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 654; /note=Magnesium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 704; /note=Magnesium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00408; METAL 857; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450; METAL 861; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450; METAL 885; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450; METAL 888; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU00450 |
| Rhea ID | RHEA:10248; RHEA:22508 |
| Cross Reference Brenda | 3.4.23.B4; |