| IED ID | IndEnz0002010500 |
| Enzyme Type ID | protease010500 |
| Protein Name |
Polybia-CP Pol-CP-NH2 Polybia chemotactic peptide |
| Gene Name | |
| Organism | Polybia paulista (Neotropical social wasp) (Swarm-founding polistine wasp) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Hymenoptera Apocrita (wasps ants and bees) Aculeata Vespoidea Vespidae (wasps) Polistinae (paper wasps) Epiponini Polybia Polybia paulista (Neotropical social wasp) (Swarm-founding polistine wasp) |
| Enzyme Sequence | ILGTILGLLKSL |
| Enzyme Length | 12 |
| Uniprot Accession Number | P0C1R0 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Potent antimicrobial peptide that is active against both Gram-positive and Gram-negative bacteria (B.subtilis (MIC=15 ug/ml or 4-16 uM), S.epidermidis (MIC=8-16 uM), S.aureus (MIC=15 ug/ml or 4 uM), E.coli (MIC=8-64 uM) and P.aeruginosa (MIC=64-128 uM)) (PubMed:16129513, PubMed:22450985, PubMed:23836163, PubMed:30534613). Also shows cytotoxicity towards HEK293 cells (32 uM) (PubMed:30534613). Adopts an amphipathic alpha helical conformation, that may allow to partition into the target membrane (PubMed:21745529, PubMed:23836163). Acts by disrupting bacterial membrane (PubMed:21745529, PubMed:22450985). Also acts in inflammation since it is chemotactic for polymorphonucleated leukocytes (PMNL) (PubMed:16129513). Shows potent antitumor activity against prostate and bladder cancer cell lines (PubMed:21745529). Causes a reduced hemolysis to mammalian erythrocytes (HC(50)=50 uM) and has no mast cell degranulation activity at physiological concentrations (PubMed:16129513, PubMed:30534613). In addition, when tested in vitro on the parasite Trypanosoma cruzi (responsible of the Chagas disease), is able to reduce the number of the three forms (epimastigote, trypomastigote and amastigote), probably acting through the apoptotic cell death pathway (PubMed:32360153). {ECO:0000269|PubMed:16129513, ECO:0000269|PubMed:21745529, ECO:0000269|PubMed:22450985, ECO:0000269|PubMed:23836163, ECO:0000269|PubMed:30534613, ECO:0000269|PubMed:32360153}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Is stable between 20 and 100 degrees Celsius. {ECO:0000269|PubMed:22450985}; |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Modified residue (1); Mutagenesis (9); Peptide (1) |
| Keywords | Amidation;Antibiotic;Antimicrobial;Chemotaxis;Direct protein sequencing;Membrane;Pharmaceutical;Secreted;Target cell membrane;Target membrane |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16129513}. Target cell membrane {ECO:0000269|PubMed:21745529, ECO:0000269|PubMed:22450985}. |
| Modified Residue | MOD_RES 12; /note=Leucine amide; /evidence=ECO:0000269|PubMed:16129513 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 1,241 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |