| IED ID | IndEnz0002010547 |
| Enzyme Type ID | protease010547 |
| Protein Name |
Lysosomal acid phosphatase LAP EC 3.1.3.2 |
| Gene Name | ACP2 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MAGKRSGWSRAALLQLLLGVNLVVMPPTRARSLRFVTLLYRHGDRSPVKTYPKDPYQEEEWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNGMQRFNPNISWQPIPVHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNESSRNAQFLDMVANETGLTDLTLETVWNVYDTLFCEQTHGLRLPPWASPQTMQRLSRLKDFSFRFLFGIYQQAEKARLQGGVLLAQIRKNLTLMATTSQLPKLLVYSAHDTTLVALQMALDVYNGEQAPYASCHIFELYQEDSGNFSVEMYFRNESDKAPWPLSLPGCPHRCPLQDFLRLTEPVVPKDWQQECQLASGPADTEVIVALAVCGSILFLLIVLLLTVLFRMQAQPPGYRHVADGEDHA |
| Enzyme Length | 423 |
| Uniprot Accession Number | P11117 |
| Absorption | |
| Active Site | ACT_SITE 42; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 287; /note=Proton donor; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a phosphate monoester + H2O = an alcohol + phosphate; Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879, ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2; |
| DNA Binding | |
| EC Number | 3.1.3.2 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (2); Chain (1); Disulfide bond (3); Glycosylation (8); Natural variant (3); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Alternative splicing;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Membrane;Reference proteome;Signal;Transmembrane;Transmembrane helix |
| Interact With | Q86V38; Q9UQC2; Q92876; Q13153 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000269|PubMed:2684640, ECO:0000269|PubMed:3056714}; Single-pass membrane protein {ECO:0000255}; Lumenal side {ECO:0000305|PubMed:2684640, ECO:0000305|PubMed:3056714}. Lysosome lumen. Note=The soluble form arises by proteolytic processing of the membrane-bound form. {ECO:0000269|PubMed:2684640, ECO:0000269|PubMed:3056714}. |
| Modified Residue | |
| Post Translational Modification | PTM: The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen. {ECO:0000269|PubMed:2684640, ECO:0000269|PubMed:3056714}.; PTM: N-glycosylated. The intermediates formed during enzymatic deglycosylation suggest that all eight predicted N-glycosylation sites are used. {ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:3056714}. |
| Signal Peptide | SIGNAL 1..30 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20217867; 21888893; |
| Motif | |
| Gene Encoded By | |
| Mass | 48,344 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15017 |
| Cross Reference Brenda |