| IED ID | IndEnz0002010581 |
| Enzyme Type ID | protease010581 |
| Protein Name |
Vacuolar membrane protease EC 3.4.-.- FXNA-related family protease 1 |
| Gene Name | BC1G_08418 |
| Organism | Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Leotiomycetes Helotiales Sclerotiniaceae Botrytis Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea) Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) |
| Enzyme Sequence | MKCYNPSAFVPMAVTLVTVIIYLGVFIPLLIIQETVPSAPDDPTLYNGLNLTEAWLDLQSLSDGYHPFNSRKNDDVRNWLLKRIGEILDHNQIQYSTETESATISETQIKSDFDAEAPESVPSPSNSNDGSAERYHEDLRARKESPAVTVFNDLRSNYSSNALTSIGVKGRKLGISTYFEGNNIICYVRGSDDEEGEWWKTGSVNSKGKVHGKGGVMVNAHFDSVSTGFGATDDGVGVVTALQLIRYFTTPQNVPQKGFVALFNNGEEDGLYGAKAFLSHPMARFVHTFLNLEGAGAGGRATLFRSTDSEVTRAYAHAKHPFGTVVSSDGFSLGYVRSETDYVVFRAEGYRGLDVAFWQPRSQYHTDQDDAKHTSIDSLWHMLSASVATTRSLTKDTGNTFLGPRGDDKVGKVSNGKGSDGVWFDIFGTVFAVFKLRTLFAWSLTLLIAAPLMLFAVSYLLNRQDKFYFFAGSIKAKGPEDEPISLGGWRGAFRYPITLIITCAITFGCASLINKINPMIVYSSPYSVWSMSASLFFSIFWFIMAGCNFVRPSALQRGYAFMWLFVFGWIILVAATVYEDRFKISGGYLFVFYEAAIFLATLIAIGEQFALPKKSTYVENSQLDHDGNQDSHHQAVMGTDGANGADEANASNEAGQEEDPEDNVNDLAVPHETTPLIGGGAPTQRSSISTTFANRYRQVVPESYDGPADHHDKKGHKKHPYGGEQDWSAKLPIWTWLVQYLLVGPFILIVVGQVGLFLVAALHQTGTDGSPLLLPYLVVAVFSILLLLPVTPFIHRLTHHMPTFFFLVFIGTLIYNLVAFPFSPNNRYKAYFQQTVDLDTGLNKVTIVGIEQYIREIIAYIPSAAGQTINCEENPRIRSGLSFCSYEGIAPRVVDNVVDGIPPEKGYTDWLTYNVTRVPNENKATFHISGLETRACILRFDDPFSEFKVHGGAKTEERWDDVPDSGSDQIKLWSRDWNKEWKVDVEWAVGEDMKPGEEGRTGRVVCLWSDANERGVIPALDEARRFSPDWTSVVKLMDGLVEGSKRRYLDEREVLEFLKVTGGVIES |
| Enzyme Length | 1067 |
| Uniprot Accession Number | A6S8A1 |
| Absorption | |
| Active Site | ACT_SITE 267; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:P80561 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: May be involved in vacuolar sorting and osmoregulation. {ECO:0000250|UniProtKB:P38244}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Glycosylation (3); Metal binding (6); Region (2); Site (1); Topological domain (10); Transmembrane (9) |
| Keywords | Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Transmembrane;Transmembrane helix;Vacuole;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250|UniProtKB:P38244}; Multi-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 118,502 |
| Kinetics | |
| Metal Binding | METAL 221; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 233; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 233; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 268; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 293; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:P80561; METAL 365; /note=Zinc 2; catalytic; /evidence=ECO:0000250|UniProtKB:P80561 |
| Rhea ID | |
| Cross Reference Brenda |