| IED ID | IndEnz0002010683 |
| Enzyme Type ID | protease010683 |
| Protein Name |
Securin Pituitary tumor-transforming gene 1 protein |
| Gene Name | Pttg1 Pttg |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MATLIFVDKDNEEPGRRLASKDGLKLGTGVKALDGKLQVSTPRVGKVFNAPAVPKASRKALGTVNRVAEKPMKTGKPLQPKQPTLTGKKITEKSTKTQSSVPAPDDAYPEIEKFFPFNPLDFESFDLPEEHQISLLPLNGVPLMTLNEERGLEKLLHLGPPSPLKTPFLSWESDPLYSPPSALSTLDVELPPVCYDADI |
| Enzyme Length | 199 |
| Uniprot Accession Number | Q9CQJ7 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of p53/TP53. The negative regulation of p53/TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (2); Chain (1); Compositional bias (1); Initiator methionine (1); Modified residue (2); Motif (4); Mutagenesis (1); Region (2); Sequence conflict (7) |
| Keywords | Acetylation;Alternative splicing;Cell cycle;Cell division;Chromosome partition;Cytoplasm;DNA damage;DNA repair;Mitosis;Nucleus;Phosphoprotein;Proto-oncogene;Reference proteome;Repeat;SH3-binding;Ubl conjugation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. |
| Modified Residue | MOD_RES 2; /note="N-acetylalanine"; /evidence="ECO:0000250|UniProtKB:O95997"; MOD_RES 162; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:19144319, ECO:0007744|PubMed:21183079" |
| Post Translational Modification | PTM: Phosphorylated at Ser-162 by CDC2 during mitosis. {ECO:0000250}.; PTM: Phosphorylated in vitro by ds-DNA kinase. {ECO:0000250}.; PTM: Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10 (By similarity). {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10650958; 10725249; 11217851; 11516952; 11682618; 12466851; 12626748; 12904583; 14561405; 14593421; 14610273; 15281346; 15919720; 15937080; 16030258; 16322543; 16355229; 16420439; 16533944; 16533945; 16602821; 16720305; 16731783; 16959877; 17039256; 17065388; 17127711; 17234588; 17325031; 17626243; 17652795; 17975001; 18232736; 18270576; 18364698; 18417614; 18639540; 18973680; 18981426; 19124608; 19213844; 19477929; 20059953; 20116377; 20153804; 20360068; 21076990; 21156286; 21163256; 21203837; 21267068; 21494564; 21677750; 21991377; 22342753; 22535767; 23164239; 23676271; 23677169; 23861251; 23867215; 24194600; 24395789; 24927528; 25421944; 26731471; 27524416; 28516917; 28658604; 29184203; 30259712; 31073078; 33181265; 34050187; 34262048; 34463328; 34571959; |
| Motif | MOTIF 58..61; /note=D-box; MOTIF 68..70; /note=TEK-box 1; MOTIF 91..93; /note=TEK-box 2; MOTIF 179..192; /note=SH3-binding |
| Gene Encoded By | |
| Mass | 21,725 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |