| IED ID | IndEnz0002010740 |
| Enzyme Type ID | protease010740 |
| Protein Name |
Zinc metalloproteinase/disintegrin Cleaved into: Snake venom metalloproteinase jararafibrase-2 SVMP EC 3.4.24.- Jararafibrase II ; Disintegrin bothrostatin D-BTT |
| Gene Name | |
| Organism | Bothrops jararaca (Jararaca) (Bothrops jajaraca) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Bothrops Bothrops jararaca (Jararaca) (Bothrops jajaraca) |
| Enzyme Sequence | MIEVLLVTICLAAFPYQGSSIILESGNVNDYEVIYPRKVTALPKGAVQPKYEDAMQYELKVNGEPVVLHLEKNKGLFSKDYSETHYSPDGRKITTNPPVEDHCYYHGRIENDADSTASISACNGLKGHFKLQGETYLIEPLKLSDSEAHAVFKFENVEKEDEAPKMCGVTQNWESYEPIKKASQSNLTPEHQRYIELFLVVDHGMFMKYNGNSDKIRRRIHQMVNIMKEAYRYLYIDIALTGVEIWSNKDMINVQPAAPQTLDSFGEWRKTDLLNRKSHDNAQLLTSTDFKDQTIGLAYWGSMCDPKRSTAVIEDHSETDLLVAVTMAHELGHNLGIRHDTGSCSCGGYSCIMAPVISHDIAKYFSDCSYIQCWDFIMKDNPQCILNKQLRTDTVSTPVSGKNFGAGEECDCGTPGNPCCDAVTCKLRPGAQCAEGLCCDQCRFMKEGTVCRRARGDDMDDYCNGISAGCPRNPFHA |
| Enzyme Length | 477 |
| Uniprot Accession Number | Q98SP2 |
| Absorption | |
| Active Site | ACT_SITE 330; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by 1,10-phenanthroline and EDTA. {ECO:0000269|PubMed:1523677}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: [Snake venom metalloproteinase jararafibrase-2]: Impairs hemostasis in the envenomed animal (By similarity). Does not exhibit detectable plasminogen activating activity. Has hemagglutinating activity on red blood cells. Cleaves insulin B chain at '38-Ala-|-Leu-39' and '40-Tyr-|-Leu-41' bonds (PubMed:8087204). {ECO:0000250, ECO:0000269|PubMed:1523677, ECO:0000269|PubMed:15737609, ECO:0000269|PubMed:8087204}.; FUNCTION: [Disintegrin bothrostatin]: This recombinant protein shows high inhibitory activity on collagen-induced platelet aggregation. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (2); Disulfide bond (9); Domain (2); Metal binding (7); Motif (1); Propeptide (2); Sequence conflict (2); Signal peptide (1) |
| Keywords | Calcium;Cell adhesion impairing toxin;Direct protein sequencing;Disulfide bond;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Platelet aggregation inhibiting toxin;Protease;Secreted;Signal;Toxin;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 455..457; /note=Cell attachment site |
| Gene Encoded By | |
| Mass | 53,440 |
| Kinetics | |
| Metal Binding | METAL 196; /note=Calcium; /evidence=ECO:0000250; METAL 280; /note=Calcium; /evidence=ECO:0000250; METAL 329; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 333; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 339; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 384; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 387; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |