| IED ID | IndEnz0002010769 |
| Enzyme Type ID | protease010769 |
| Protein Name |
Presequence protease 2, chloroplastic/mitochondrial AtPreP2 PreP 2 EC 3.4.24.- Zinc metalloprotease 2 AtZnMP2 |
| Gene Name | PREP2 ZNMP2 At1g49630 F14J22.13 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MLRSLTCSSTITSTSLFFRSFRQLPRSYLSPSSSTTVVGASGRNIRRLSTLEAAGRRLFLRRGLKLLSAASRGLNGQFSRLSIRAVATQSAPSSYPGQDEAEKLGFEKVSEEFISECKSKAVLFKHKKTGCEVMSVSNDDENKVFGIVFRTPPKDSTGIPHILEHSVLCGSRKYPMKEPFVELLKGSLHTFLNAFTYPDRTCYPVASTNKKDFYNLVDVYLDAVFFPKCVDDVHTFQQEGWHYELNDPSEDISYKGVVFNEMKGVYSQPDNILGRVTQQALCPENTYGVDSGGDPKDIPKLTFEKFKEFHRQYYHPSNARIWFYGDDDPVHRLRVLSEYLDMFDASPARDSSKVEPQKLFSRPRRIVEKYPAGEDGDLKKKHMVCLNWLLSDKPLDLQTQLALGFLDHLMLGTPASPLRKILLESGLGEALVNSGMEDELLQPQFSIGLKGVSDDNVQKVEELVMNTLRKLADEGFDTDAVEASMNTIEFSLRENNTGSSPRGLSLMLQSIAKWIYDMDPFEPLKYEEPLKSLKARIAEKGSKSVFSPLIEEYILNNPHCVTIEMQPDPEKASLEEAEEKSILEKVKASMTEEDLTELARATEELRLKQETPDPPDALKCVPSLNLSDIPKEPIYVPTEVGDINGVKVLRNDLFTNNILYTEVVFDMGSVKHELLQLIPLFCQSLLEMGTQDLTFVQLNQLIGRKTGGISVYPLTSSVYGRDDPCSKIIVRGKSMVGRAEDLFNLMNCVLQEVRFTDQQRFKQFVSQSRARMENRLRGSGQGIAAARMDAMLNVAGWMSEQMGGLSYLEFLHTLEQKVDQDWEGISSSLEEIRRSFLSRNGCIVNMTADGKSLTNTEKYVGKFLDLLPENPSGELVTWDARLPLRNEAIVIPTQVNYVGKAGNIYSSGYKLDGSSYVISKHISNTWLWDRVRVSGGAYGGSCDFDSHSGVFSFLSYRDPNLLKTLDIYDGTGDFLRGLDVDEDTLTKAIIGTIGDVDSYQLPDAKGYTSLLRHLLNVTDEERQIRREEILSTSLKDFKEFAEAIDSVSDKGVAVAVASQEDIDAANRERSNFFEVKKAAL |
| Enzyme Length | 1080 |
| Uniprot Accession Number | Q8VY06 |
| Absorption | |
| Active Site | ACT_SITE 164; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:Q9LJL3; ACT_SITE 239; /evidence=ECO:0000250|UniProtKB:Q9LJL3 |
| Activity Regulation | ACTIVITY REGULATION: Completely inhibited by the metal chelator orthophenanthroline. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: ATP-independent protease that degrades both mitochondrial and chloroplastic transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. {ECO:0000269|PubMed:12138166}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous gene model prediction (1); Metal binding (3); Transit peptide (1) |
| Keywords | Chloroplast;Hydrolase;Magnesium;Metal-binding;Metalloprotease;Mitochondrion;Plastid;Protease;Reference proteome;Transit peptide;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:15827031}. Mitochondrion matrix {ECO:0000269|PubMed:15827031}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11826309; 14576160; 14617063; 16766689; 17081117; 18230142; 18431481; 19701724; 21621546; 21995547; 24373893; 28627464; 30242930; 32663165; |
| Motif | |
| Gene Encoded By | |
| Mass | 121,131 |
| Kinetics | |
| Metal Binding | METAL 161; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q9LJL3; METAL 165; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q9LJL3; METAL 261; /note=Zinc; /evidence=ECO:0000250|UniProtKB:Q9LJL3 |
| Rhea ID | |
| Cross Reference Brenda |