| IED ID | IndEnz0002010824 |
| Enzyme Type ID | protease010824 |
| Protein Name |
Suppressor of tumorigenicity 14 protein homolog EC 3.4.21.109 Epithin Serine protease 14 |
| Gene Name | St14 Prss14 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MGSNRGRKAGGGSQDFGAGLKYNSRLENMNGFEEGVEFLPANNAKKVEKRGPRRWVVLVAVLFSFLLLSLMAGLLVWHFHYRNVRVQKVFNGHLRITNEIFLDAYENSTSTEFISLASQVKEALKLLYNEVPVLGPYHKKSAVTAFSEGSVIAYYWSEFSIPPHLAEEVDRAMAVERVVTLPPRARALKSFVLTSVVAFPIDPRMLQRTQDNSCSFALHAHGAAVTRFTTPGFPNSPYPAHARCQWVLRGDADSVLSLTFRSFDVAPCDEHGSDLVTVYDSLSPMEPHAVVRLCGTFSPSYNLTFLSSQNVFLVTLITNTDRRHPGFEATFFQLPKMSSCGGFLSDTQGTFSSPYYPGHYPPNINCTWNIKVPNNRNVKVRFKLFYLVDPNVPVGSCTKDYVEINGEKYCGERSQFVVSSNSSKITVHFHSDHSYTDTGFLAEYLSYDSNDPCPGMFMCKTGRCIRKELRCDGWADCPDYSDERYCRCNATHQFTCKNQFCKPLFWVCDSVNDCGDGSDEEGCSCPAGSFKCSNGKCLPQSQKCNGKDNCGDGSDEASCDSVNVVSCTKYTYRCQNGLCLSKGNPECDGKTDCSDGSDEKNCDCGLRSFTKQARVVGGTNADEGEWPWQVSLHALGQGHLCGASLISPDWLVSAAHCFQDDKNFKYSDYTMWTAFLGLLDQSKRSASGVQELKLKRIITHPSFNDFTFDYDIALLELEKSVEYSTVVRPICLPDATHVFPAGKAIWVTGWGHTKEGGTGALILQKGEIRVINQTTCEDLMPQQITPRMMCVGFLSGGVDSCQGDSGGPLSSAEKDGRMFQAGVVSWGEGCAQRNKPGVYTRLPVVRDWIKEHTGV |
| Enzyme Length | 855 |
| Uniprot Accession Number | P56677 |
| Absorption | |
| Active Site | ACT_SITE 656; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 711; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 805; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleaves various synthetic substrates with Arg or Lys at the P1 position and prefers small side-chain amino acids, such as Ala and Gly, at the P2 position.; EC=3.4.21.109; |
| DNA Binding | |
| EC Number | 3.4.21.109 |
| Enzyme Function | FUNCTION: Degrades extracellular matrix. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site (By similarity). Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (18); Domain (8); Glycosylation (6); Modified residue (1); Topological domain (2); Transmembrane (1) |
| Keywords | Disulfide bond;Glycoprotein;Hydrolase;Membrane;Phosphoprotein;Protease;Reference proteome;Repeat;Serine protease;Signal-anchor;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}. |
| Modified Residue | MOD_RES 13; /note=Phosphoserine; /evidence=ECO:0007744|PubMed:21183079 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11567025; 12032844; 12466851; 12520002; 14610273; 14638864; 14747469; 15629466; 15848395; 16103220; 16141072; 16170303; 16428450; 16524950; 16651618; 16980306; 16983341; 17174946; 17228523; 17328885; 17471493; 17940283; 17967808; 18402552; 18554416; 18713750; 18799693; 18832587; 19156209; 19387477; 19389929; 19592578; 19717635; 19853659; 20142489; 20152175; 20398629; 20652801; 20657595; 21149451; 21217780; 21267068; 22081509; 22139080; 22514623; 22952456; 24194600; 24469043; 24551030; 24685580; 24962579; 25078604; 25245289; 25486433; 25873032; 26599507; 27385010; 28094766; 28490634; 30559294; 31628112; 32907879; |
| Motif | |
| Gene Encoded By | |
| Mass | 94,655 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |