| IED ID | IndEnz0002010849 |
| Enzyme Type ID | protease010849 |
| Protein Name |
Vacuolar-processing enzyme alpha-isozyme EC 3.4.22.34 Alpha-VPE Asparaginyl endopeptidase alpha-VPE |
| Gene Name | At2g25940 T19L18.25 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MTTVVSFLALFLFLVAAVSGDVIKLPSLASKFFRPTENDDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRPGVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQYELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFVNENSIRPPSRVTNQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFGLEGHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMRQMEEAAMQACPTIPTSPWSSLDRGFSA |
| Enzyme Length | 478 |
| Uniprot Accession Number | P49047 |
| Absorption | |
| Active Site | ACT_SITE 162; /evidence=ECO:0000255; ACT_SITE 204; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.; EC=3.4.22.34; Evidence={ECO:0000250|UniProtKB:Q84LM2}; |
| DNA Binding | |
| EC Number | 3.4.22.34 |
| Enzyme Function | FUNCTION: Asparagine-specific endopeptidase involved in the processing of vacuolar seed protein precursors into the mature forms (PubMed:7579169). Probably involved in post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds (PubMed:14688293). {ECO:0000269|PubMed:14688293, ECO:0000269|PubMed:7579169}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Signal peptide (1); Site (1) |
| Keywords | Hydrolase;Protease;Reference proteome;Signal;Thiol protease;Vacuole |
| Interact With | |
| Induction | INDUCTION: By senescence, wounding, ethylene and salicylic acid (SA). {ECO:0000269|PubMed:10417725}. |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:7579169}. |
| Modified Residue | |
| Post Translational Modification | PTM: Auto-catalytic activation. {ECO:0000250|UniProtKB:Q84LM2}. |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17059406; 18796636; 22822203; 8589932; |
| Motif | |
| Gene Encoded By | |
| Mass | 52,670 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |