| IED ID | IndEnz0002010865 |
| Enzyme Type ID | protease010865 |
| Protein Name |
Zinc metalloproteinase-disintegrin-like VAP1 EC 3.4.24.- Snake venom metalloproteinase SVMP Vascular apoptosis-inducing protein 1 Vap-1 |
| Gene Name | |
| Organism | Crotalus atrox (Western diamondback rattlesnake) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Crotalus Crotalus atrox (Western diamondback rattlesnake) |
| Enzyme Sequence | MIQVLLVTISLAVFPYQGSSVILESGNVNDYEVVYPRKVTALPKGAVQPKYEDAMQYEFKVNGEPVVLHLEKNKGLFSEDYSETHYSPDGREITTYPPVEDHCYYHGRIENDADSTASISACNGLKGHFKLQGEMYLIEPLKLPDSEAHAVFKYENVEKEDEAPKMCGVTQNWESYEPIKKASQSNLTPEQQRYLNAKKYVKLFLVADYIMYLKYGRNLTAVRTRMYDIVNVITPIYHRMNIHVALVGLEIWSNTDKIIVQSSADVTLDLFAKWRATDLLSRKSHDNAQLLTGINFNGPTAGLGYLGGICNTMYSAGIVQDHSKIHHLVAIAMAHEMGHNLGMDHDKDTCTCGTRPCVMAGALSCEASFLFSDCSQKDHREFLIKNMPQCILKKPLKTDVVSPAVCGNYFVEVGEECDCGSPRTCRDPCCDATTCKLRQGAQCAEGLCCDQCRFKGAGTECRAAKDECDMADVCTGRSAECTDRFQRNGQPCKNNNGYCYNGKCPIMADQCIALFGPGATVSQDACFQFNREGNHYGYCRKEQNTKIACEPQDVKCGRLYCFPNSPENKNPCNIYYSPNDEDKGMVLPGTKCADRKACSNGQCVDVTTPY |
| Enzyme Length | 610 |
| Uniprot Accession Number | Q9DGB9 |
| Absorption | |
| Active Site | ACT_SITE 336; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by EDTA and EGTA, but not by PMSF. {ECO:0000269|PubMed:11071872}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Zinc metalloprotease that has fibrinogenolytic and hemorrhagic activities. It induces apoptosis in vascular endothelial cells (VEC), without degrading extracellular matrix (it cannot cleave collagen) or inhibiting adhesion of VEC. VAP1-induced apoptosis is inhibited by antibodies for integrin alpha-3, alpha-6, beta-1 and CD9. Apoptosis is accompanied by severe cell fragmentation, which is controlled by caspases. {ECO:0000269|PubMed:11071872, ECO:0000269|PubMed:11821125, ECO:0000269|PubMed:15922392, ECO:0000269|PubMed:18657564, ECO:0000269|PubMed:9196035}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (16); Chain (1); Disulfide bond (18); Domain (2); Glycosylation (1); Helix (10); Metal binding (14); Modified residue (1); Motif (1); Propeptide (1); Signal peptide (1); Turn (9) |
| Keywords | 3D-structure;Apoptosis;Calcium;Cell adhesion impairing toxin;Direct protein sequencing;Disulfide bond;Fibrinogenolytic toxin;Glycoprotein;Hemorrhagic toxin;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Protease;Pyrrolidone carboxylic acid;Secreted;Signal;Toxin;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | MOD_RES 190; /note=Pyrrolidone carboxylic acid (Glu); /evidence=ECO:0000250|UniProtKB:P0DM89 |
| Post Translational Modification | PTM: The N-terminus is blocked. {ECO:0000269|PubMed:19371136}. |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 2ERO; 2ERP; 2ERQ; |
| Mapped Pubmed ID | - |
| Motif | MOTIF 467..469; /note=D/ECD-tripeptide |
| Gene Encoded By | |
| Mass | 67,960 |
| Kinetics | |
| Metal Binding | METAL 335; /note=Zinc; catalytic; METAL 339; /note=Zinc; catalytic; METAL 345; /note=Zinc; catalytic; METAL 405; /note=Calcium 1; via carbonyl oxygen; METAL 408; /note=Calcium 1; METAL 410; /note=Calcium 1; via carbonyl oxygen; METAL 412; /note=Calcium 1; METAL 415; /note=Calcium 1; METAL 418; /note=Calcium 1; METAL 469; /note=Calcium 2; METAL 470; /note=Calcium 2; via carbonyl oxygen; METAL 472; /note=Calcium 2; METAL 483; /note=Calcium 2; METAL 484; /note=Calcium 2; via carbonyl oxygen |
| Rhea ID | |
| Cross Reference Brenda |