| IED ID | IndEnz0002010876 |
| Enzyme Type ID | protease010876 |
| Protein Name |
Zinc metalloproteinase-disintegrin-like uracoina-1 EC 3.4.24.- Snake venom metalloprotease SVMP Fragment |
| Gene Name | |
| Organism | Crotalus vegrandis (Uracoan rattlesnake) (Crotalus durissus vegrandis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Lepidosauria (lepidosaurs) Squamata (squamates) Bifurcata (split-tongued squamates) Unidentata Episquamata Toxicofera Serpentes (snakes) Colubroidea Viperidae Crotalinae (pit vipers) Crotalus Crotalus vegrandis (Uracoan rattlesnake) (Crotalus durissus vegrandis) |
| Enzyme Sequence | HQKYNPFRFVELVLVVDKAM |
| Enzyme Length | 20 |
| Uniprot Accession Number | C0HL97 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by ethylenediaminetetraacetic acid (EDTA) and 1,10-phenanthroline. Not inhibited by tosyl-L-lysine chloromethyl ketone (TCLK) and phenylmethanesulfonylfluoride (PMSF). {ECO:0000269|PubMed:11451438}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Snake venom zinc metalloprotease that possesses hemorrhagic activity (minimum hemorrhagic dose, MHD=4.7 ug) when injected intradermally into mice. Degrades the alpha-chain of fibrinogen (FGA). {ECO:0000269|PubMed:11451438}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Activity is stable between 20-40 degrees Celsius, decreases at higher temperatures and is lost at 70 degrees Celsius. {ECO:0000269|PubMed:11451438}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7-9. {ECO:0000269|PubMed:11451438}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Non-terminal residue (1) |
| Keywords | Direct protein sequencing;Fibrinogenolytic toxin;Hemorrhagic toxin;Hemostasis impairing toxin;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Toxin;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11451438}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 2,434 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |