| IED ID | IndEnz0002010913 |
| Enzyme Type ID | protease010913 |
| Protein Name |
Prohead protease EC 3.4.21.- |
| Gene Name | T5.150 T5p146 |
| Organism | Escherichia phage T5 (Enterobacteria phage T5) |
| Taxonomic Lineage | Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes Caudovirales Demerecviridae Markadamsvirinae Tequintavirus Escherichia phage T5 (Enterobacteria phage T5) |
| Enzyme Sequence | MTQAAIDYNKLKSAPVHLDAYIKSIDSESKEGVVKIRGFANTISKDRAGDVIPASAWKTSNALTNYMKNPIILFGHDHRRPIGKCIDLNPTEMGLEIECEINESSDPAIFSLIKNGVLKTFSIGFRCLDAEWDEATDIFIIKDLELYEVSVVSVPCNQDSTFNLAKSMNGHDYTEWRKSFTAISSKAVPAQERNLSELEKLAIALGYVKE |
| Enzyme Length | 210 |
| Uniprot Accession Number | Q6QGD7 |
| Absorption | |
| Active Site | ACT_SITE 76; /evidence=ECO:0000269|PubMed:26616586; ACT_SITE 122; /evidence=ECO:0000269|PubMed:26616586; ACT_SITE 148; /evidence=ECO:0000269|PubMed:26616586 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease involved in capsid assembly and maturation. Cleaves the major capsid protein, the decoration protein, the portal protein to yield mature procapsids competent for DNA packaging (Probable). Acts as a trigger for assembly of the capsid protein. {ECO:0000269|PubMed:24198424, ECO:0000269|PubMed:26616586}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Mutagenesis (3); Propeptide (2); Sequence conflict (2); Site (2) |
| Keywords | Hydrolase;Late protein;Protease;Reference proteome;Serine protease;Viral capsid assembly;Viral capsid maturation;Viral release from host cell;Virion |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:24198424}. Note=Present in the prohead I. {ECO:0000269|PubMed:24198424}. |
| Modified Residue | |
| Post Translational Modification | PTM: Cleaves itself autocatalytically to yield the mature form of the protease (Probable). {ECO:0000269|PubMed:24198424}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 23,379 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |