| IED ID | IndEnz0002010977 |
| Enzyme Type ID | protease010977 |
| Protein Name |
Ovochymase-2 EC 3.4.21.120 Oviductal protease Oviductin |
| Gene Name | OVCH2 OVTN |
| Organism | Rhinella arenarum (Argentine common toad) (Bufo arenarum) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Neobatrachia Hyloidea Bufonidae (true toads) Rhinella (beaked toads) Rhinella arenarum (Argentine common toad) (Bufo arenarum) |
| Enzyme Sequence | MAETSVFSIMMLTVMTAVGRGATDRPGRVSRCGERPSANASVTYNLLSRIVGGTSAVKGESPWMVSLKRDGKHFCGGTIISDKHVLTAAHCVLDKNIEYHVRVSIGDHDFTVYERSEQIFAIKAVFKHPNFNPIRPFNYDLAIVELGESIAFDKDIQPACLPSPDDVFPTGTLCIALGWGRLQENGRLPSSLQQVVLPLIEYRKCLSIMETVDRRLAFETVVCAGFPEGGKDACQGDSGGPFLCQRSQGRWVLVGVTSWGLGCARKWVDNILDPPERRGSPGVFTDIQRLLNWLSANLNQDKPDFPTYEVQCSTNDGIEKGTAGEILLPTDYRKYYSNNEKCIWTIIVPKGKHILLTFNSFNVEWDYSCDLDYLVIYSALGRLIGKFCGDVRPRPLLIADASVTLKFISDFQEYKTGFSLFYQAVEPNTYPDSDCGSVAVIFEEGEIQTMNHPHVYSSHANCQWVVHSPANHIIKITFLVFEVEPSEGCIFDRLVVYHDLQGTMVAGIFCGFSLPDPVLSVSNVMQITFTSDYSVNYLGFQAVISFVLPSSPVKSETQWKGNNQPRKNQDAMQHYEEGCGVSPLPPRFIHHNIIKAEEAMPNSWPWHVSINFGNKHLCNGAILSKTFVVTSANCVADREEFPSVGLIVAGLHDLESSTDAQKRTVEYVIVHPDYNRLSKDYDVALIHVQMPFQYNSHVQPICLPDGHSKLEPSKLCVVSGWDLNVELSTKLQQLEVPVLMDDVCKKYYDGITDRMFCAGVIAEEDNVSCLAQSGAPLVCQSDPGTYVIFGIVSWGVGCNEPPKAGVYSSVPLFIPWIMETILSVAGIANTDTEPHHPLIPPDKLSQEKALLPDSPPSNDSSSSQDIYVTCKDVMSLQSPGEIKLVASAQDGPEGGRCQLIFQAPEDHFILLHFKQLSHEHYSLIIYEGASSNKTFKAQLTEEKIPTIMKSVGPVITIEASSTAQDSALHLWLSYSFHNQN |
| Enzyme Length | 980 |
| Uniprot Accession Number | Q66TN7 |
| Absorption | |
| Active Site | ACT_SITE 90; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 140; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 238; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage at 371-Gly-Ser-Arg-|-Trp-374 of glycoprotein gp43 in Xenopus laevis coelemic egg envelope to yield gp41.; EC=3.4.21.120; Evidence={ECO:0000250|UniProtKB:Q90WD8}; |
| DNA Binding | |
| EC Number | 3.4.21.120 |
| Enzyme Function | FUNCTION: Mediates gamete interaction by affecting the vitelline coat. {ECO:0000250|UniProtKB:Q90WD8}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (12); Domain (4); Glycosylation (4); Metal binding (2); Propeptide (2); Region (1); Signal peptide (1) |
| Keywords | Calcium;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Protease;Repeat;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P79953}. |
| Modified Residue | |
| Post Translational Modification | PTM: The catalytically inactive 108 kDa form is processed both N- and C-terminally to give rise to catalytically active and inactive forms. {ECO:0000305|PubMed:20810007}. |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 108,566 |
| Kinetics | |
| Metal Binding | METAL 112; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 117; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.120; |