| IED ID | IndEnz0002010978 |
| Enzyme Type ID | protease010978 |
| Protein Name |
Ovochymase-2 EC 3.4.21.120 Oviductal protease Oviductin |
| Gene Name | ovch2 ovtn |
| Organism | Xenopus laevis (African clawed frog) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Xenopus Xenopus laevis (African clawed frog) |
| Enzyme Sequence | MPTRNLLLGSILLSLAVKGDPGPHRGARCGVSPLGSATELNYLSRIVGGRESKKGQHPWTVSLKRNGKHFCGGTLVSHCHVLTAAHCLLDRNVKLYMRVYIGEYDQILKEETEQMFRVIEIFKHPNFNQSQPMNYDVAVLLLDGSVTFDENIQPACLPNPDDVFEPGDLCVTLGWGHLTENGILPVVLQEVYLPIVDLSSCLHVMSALKGTVVSSYIVCAGFPEGGKDACQGDSGGPLLCQRRHGSWVLHGLTSWGMGCGRSWKNNVFLPHNRKGSPGIFTDIQKLLGWVSSQLNTAVPNKNQESCSMQDGVLSGKSGELIFLKNPMSVTRTMSGAPGFSLSLKTCTSCLNFTHLDIESDFACNLDYLAIYTDSHRLIGKFCGDIPPRSLLISFSSIKLNFFSDFHENRTGFVLYYSAVEPNTYPDSGCGSFAVLFEEGEIQSMNYPENYLSNSRCHWIIHGPSGSYIKLQFEDFALEPSDDCRSDYLAVYQDLAAEDKIETFCGFSLPAPVYSTTAVMHIKFSTDERDNDKGFRATFTFVSPNSLVEDSRQGNMPSTNKKETTAQDSICGVSQVPPIFIYNSIAKVEEAVPHSWPWHTSLQYAGEHVCDGAIIAENWILTTASCVLNRKFNDVWLVDPGIHDLLRPGHNQKGLVKQIIPHPSFSSQTNDFDIALVELDESLQFNSDIFPICLPGKTSELAPASLCVVSGWSLRGKEAEKSTKLQQREVPILTDDACSAHYIQNPGGITDRMLCAGIGTGQDNDSCSEQSGSPLVCLLEKKGIYTIFGIASWGVNCKENSKPGIYTKVSPFIDWIRQIMSDTGQIHSNLGDPKPHPMGNIEPEETAGRDIIQGGFPTNDASSNQNLYIASSCEDVVLLQSPGEIKMETKSQMYPNGFSCQWRIIAPKFQIIKLVMKQVHMSAENGKCCNSLIIYEGISKNKTLKVRFPTDEMVPGTVWSEGSSVTIESPPHPVDPEFGFCLVYSFHSRTQSQDHVVPDSDSSEP |
| Enzyme Length | 1004 |
| Uniprot Accession Number | P79953 |
| Absorption | |
| Active Site | ACT_SITE 86; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 136; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 234; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage at 371-Gly-Ser-Arg-|-Trp-374 of glycoprotein gp43 in Xenopus laevis coelemic egg envelope to yield gp41.; EC=3.4.21.120; Evidence={ECO:0000269|PubMed:10084976}; |
| DNA Binding | |
| EC Number | 3.4.21.120 |
| Enzyme Function | FUNCTION: Converts the glycoprotein envelope surrounding the egg from an unfertilizable to a fertilizable form during its transit through the pars recta portion of the oviduct by selectively hydrolyzing the envelope glycoprotein gp43. The egg envelope is converted to a sperm-penetrable form, via an increase in sperm binding. {ECO:0000269|PubMed:10084976}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (11); Domain (4); Glycosylation (5); Metal binding (1); Propeptide (2); Region (1); Signal peptide (1) |
| Keywords | Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Protease;Repeat;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10084976}. |
| Modified Residue | |
| Post Translational Modification | PTM: The catalytically inactive 110 kDa form is processed both N- and C-terminally to give rise to the 66 kDa catalytically active form. {ECO:0000269|PubMed:10084976}. |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 110,612 |
| Kinetics | |
| Metal Binding | METAL 113; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.120; |