| IED ID | IndEnz0002010979 |
| Enzyme Type ID | protease010979 |
| Protein Name |
Ovochymase-2 EC 3.4.21.120 Oviductal protease Oviductin |
| Gene Name | OVCH2 OVTN |
| Organism | Bufo japonicus (Japanese toad) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Neobatrachia Hyloidea Bufonidae (true toads) Bufo Bufo japonicus (Japanese toad) |
| Enzyme Sequence | MAETSIFPIMMLTVMIGVGRGVTDSPGRVSRCGERPAANTSVSYGLLSRIVGGTSAVKGESPWMVSLKRDGKHFCGGTIISDKYVLTAAHCVLEKNFEFQVSVSIGDHDFAVYERSEQRFAIKSVFKHPNFKPSRPFNYDLAILELVESITFDKDIQPACLPSPDDVFPTGTLCMALGWGRLQENGRLPSSLQKVVLPLIEYRRCLSIMETVDRRLAFETVVCAGFPEGGKDACQGDSGGPFLCQRSQGRWVLVGVTSWGLGCARKWADNILDPVESKGSPGVFTDIQRLLNWLSENLNQDKPDFPTYQVQCSTNDGIEKGTTGEILLPTGYKKYYSNNEKCIWTIIVPRGKHILLTFKSFNVECDYSCDLDYLVIYSALGRLIGKFCGDVSPRPLLIADASITLKFISDFHEYKTGFSLFYEAVEPDTYPDSDCGSVAVIFEEGEIQTMNHPHLYSSHANCQWVVHSPANYIIKITFLVFEVEPSEGCIFDRLVVYHDLQGTVVAGFFCGFALPDPVLSVSNVMQITFTSDYSANYLGFRAVISFVLPSSPVKPEKGNNQPRKNQDAMQHFDEGCGVSPLPPRFLYHNLIKAEEAMPNSWPWHVSINFGNKHVCNGAILSKTFVVTSANCVADREEFPSIGLIVAGLHDLESSINTQKRPVEYVIVHPDYNRLSKDYDVALIHVQRPFQYNSYVQPICLPDGHSRLEPSKLCVVSGWDLNVELSTKLQQLEVPVLMDDVCKKYYDGITDRMFCAGVIAEEDNASCLAQSGAPLVCQSAPGTYAIFGIVSRGVGCNETPKAGVYSSVFLFIPWIMETILSVAGIIDTDSEPHHPLFPPDKPSQQKALLPDSPPSSSSQDIYVTCKDVLSLQSPGEIKLVASGQDGPEGGRCQLIFQAPEGHFILLTFKQLSHEHYSLIIYEGASSNKTFKAQLMEEKIPTIMKSAGAVITLEASSTAQDSALHLWLSYSFHNQN |
| Enzyme Length | 974 |
| Uniprot Accession Number | Q90WD8 |
| Absorption | |
| Active Site | ACT_SITE 90; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 140; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 238; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage at 371-Gly-Ser-Arg-|-Trp-374 of glycoprotein gp43 in Xenopus laevis coelemic egg envelope to yield gp41.; EC=3.4.21.120; Evidence={ECO:0000269|PubMed:11846486}; |
| DNA Binding | |
| EC Number | 3.4.21.120 |
| Enzyme Function | FUNCTION: Mediates gamete interaction by affecting the vitelline coat. {ECO:0000269|PubMed:11846486}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (12); Domain (4); Glycosylation (3); Metal binding (2); Propeptide (2); Region (1); Signal peptide (1) |
| Keywords | Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Protease;Repeat;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | INDUCTION: By human chorionic gonadotropin (HCG). {ECO:0000269|PubMed:11846486}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P79953}. |
| Modified Residue | |
| Post Translational Modification | PTM: The catalytically inactive 107 kDa form is processed both N- and C-terminally to give rise to the 66 kDa catalytically active form and inactive forms of 82 kDa and 59 kDa. {ECO:0000269|PubMed:11846486}. |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 107,648 |
| Kinetics | |
| Metal Binding | METAL 112; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 117; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.120; |