| IED ID | IndEnz0002010992 |
| Enzyme Type ID | protease010992 |
| Protein Name |
pH-response transcription factor pacC/RIM101 Cleaved into: pH-response transcription factor pacC/RIM101 closed form; pH-response transcription factor pacC/RIM101 open form 1; pH-response transcription factor pacC/RIM101 open form 2 |
| Gene Name | pacC AN2855 |
| Organism | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Enzyme Sequence | MLGAMAEEAVAPVAVPTTQEQPTSQPAAAQVTTVTSPSVTATAAAATAAVASPQANGNAASPVAPASSTSRPAEELTCMWQGCSEKLPTPESLYEHVCERHVGRKSTNNLNLTCQWGSCRTTTVKRDHITSHIRVHVPLKPHKCDFCGKAFKRPQDLKKHVKTHADDSVLVRSPEPGSRNPDMMFGGNGKGYAAAHYFEPALNPVPSQGYAHGPPQYYQAHHAPQPSNPSYGNVYYALNTGPEPHQASYESKKRGYDALNEFFGDLKRRQFDPNSYAAVGQRLLSLQNLSLPVLTAAPLPEYQAMPAPVAVASGPYGGGPHPAPAYHLPPMSNVRTKNDLINIDQFLQQMQDTIYENDDNVAAAGVAQPGAHYIHNGISYRTTHSPPTQLPSAHATTQTTAGPIISNTSAHSPSSSTPALTPPSSAQSYTSGRSPISLPSAHRVSPPHESGSSMYPRLPSATDGMTSGYTAASSAAPPSTLGGIFDNDERRRYTGGTLQRARPASRAASESMDLSSDDKESGERTPKQISASLIDPALHSGSPGEDDVTRTAKAATEVAERSDVQSEWVEKVRLIEYLRNYIANRLERGEFSDDSEQEQDQEQEQDQEQEQDQEQGQDRVSRSPVSKADVDMEGVERDSLPRSPRTVPIKTDGESAEDSVMYPTLRGLDEDGDSKMPS |
| Enzyme Length | 678 |
| Uniprot Accession Number | Q00202 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Transcription factor that mediates regulation of both acid- and alkaline-expressed genes in response to ambient pH. At alkaline ambient pH, activates transcription of alkaline-expressed genes (including pacC itself) and represses transcription of acid-expressed genes. Specifically recognizes and binds the consensus sequence 5'-GCCARG-3'. Required for virulence in invasive pulmonary aspergillosis (IPA). {ECO:0000269|PubMed:15686555, ECO:0000269|PubMed:7628696, ECO:0000269|PubMed:7882981}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Compositional bias (5); Motif (3); Mutagenesis (9); Propeptide (2); Region (10); Sequence conflict (2); Zinc finger (3) |
| Keywords | Activator;Cytoplasm;DNA-binding;Metal-binding;Nucleus;Reference proteome;Repeat;Repressor;Transcription;Transcription regulation;Zinc;Zinc-finger |
| Interact With | |
| Induction | INDUCTION: By alkaline conditions. {ECO:0000269|PubMed:7882981}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11238906}. Nucleus {ECO:0000269|PubMed:11238906}. Note=Cytoplasmic at acidic ambient pH, and nuclear in its processed form at alkaline ambient pH. |
| Modified Residue | |
| Post Translational Modification | PTM: Activated by C-terminal proteolytic cleavage. At neutral to alkaline ambient pH, the signaling protease (probably palB) cleaves pacC within the conserved 24-residue signaling protease box, removing the C-terminal interacting region C and producing a 53 kDa 'open' conformation intermediate protein, which is committed to further processing. In an ambient pH-independent reaction, the processing protease (probably the proteasome) removes additional C-terminal residues to yield the 27 kDa functional form. {ECO:0000269|PubMed:11889040, ECO:0000269|PubMed:7628696}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 158..164; /note=Nuclear localization signal; MOTIF 455..458; /note=YPX[LI] motif 1; MOTIF 662..665; /note=YPX[LI] motif 2 |
| Gene Encoded By | |
| Mass | 72,939 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |