| IED ID | IndEnz0002011002 |
| Enzyme Type ID | protease011002 |
| Protein Name |
Subtilisin-like protease PIMMS2 EC 3.4.21.62 Midgut invasion ookinete protein 2 |
| Gene Name | PIMMS2 PBANKA_1106900 |
| Organism | Plasmodium berghei (strain Anka) |
| Taxonomic Lineage | cellular organisms Eukaryota Sar Alveolata Apicomplexa Aconoidasida Haemosporida (haemosporidians) Plasmodiidae Plasmodium Plasmodium (Vinckeia) Plasmodium berghei Plasmodium berghei (strain Anka) |
| Enzyme Sequence | MVLLNGKLKYIAVVAIFYNLIILLVKEKFPYICTKKKFHAISNRILYEYLNNFVSKDIFRREDITLKNLNFVQTNLKSDKDAEIKENRDTQSVDDNMFQRVYKFILNFFYGNKKNRINKSMNYGKYDNFNKINDIFEFMRNNGLPINITSVCLIDTGLNIKDALINYFLNHDISTYNSYTYHSVNINYKKPDSFNFGINSENCDEDNYSECESTFLENHNGHGKYEDKSTIQGDSLKLIEKKYDKNVDLQRSGIDVEICKAFNNSKEKKNSLNIIPVIKCLEYCKTKNVKIIHMDYNINEQNEQLIQIMDDLKNSEIFVILPSEKLFNEKPYEDNSVIYPSSFFEKFENVFFIGSLDYSDMSSDDADIASNFQIQKNEYLKYRKNNVFLLDSINSSLKKRDDHDILYYEIKYSSAFFINIITIILNIYPNMSIKELRNILSYSIPSKETAQLKTENIFEGNFDINKFIHILLNRGINSSGFVRKYKDVTPNESTNKIFLLEDQKDADIEPQKDSSIDIYCDEGGSDEDIVSTSNGLDVYSEYSHSNNKSDQLLNDEKGLKYETYKDLYSVKENDIYVFESNNPTNSSFMQMNYDDKIKSKYLDNLEEANYQNHRTQFEINRNDNRYPIISEDNLRDRQNMHNIQMLNDGINYSENANNIEELYDNDFEDYRGKDLNPEYNKIRDNNNNKNINKEFGILNTWRKNNEGLYLSDSEEESQPFKWMDMHVDDIYQDRKKRLKGNNYDNRNGRVRRIYEDNKRGRYIKNRNMQKKKNFDRNLKNKRYLNRRTKRHINEKNKRIMREKKNKYNNSVLKRNEMKSHNNSQKTPKIIPRKYSR |
| Enzyme Length | 836 |
| Uniprot Accession Number | A0A509AKI1 |
| Absorption | |
| Active Site | ACT_SITE 155; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10080; ACT_SITE 222; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P00782; ACT_SITE 414; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P00782 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.; EC=3.4.21.62; Evidence={ECO:0000250|UniProtKB:Q8I0V0}; |
| DNA Binding | |
| EC Number | 3.4.21.62 |
| Enzyme Function | FUNCTION: Probable serine protease which plays a role in ookinete traversal of the mosquito host midgut epithelium. {ECO:0000269|PubMed:28559405, ECO:0000269|PubMed:29873127}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Mutagenesis (3); Region (1) |
| Keywords | Cell membrane;Hydrolase;Membrane;Protease;Reference proteome;Serine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28559405}. Note=It is not clear how the protein localizes at the cell membrane. There is a predicted transmembrane domain encompassing the end of the catalytic domain which appears to be intracellular suggesting a single-pass type II membrane topology. {ECO:0000305|PubMed:28559405}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 99,420 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.62; |