| IED ID |
IndEnz0002011075 |
| Enzyme Type ID |
protease011075 |
| Protein Name |
Tissue inhibitor of metalloproteinase
|
| Gene Name |
Timp CG6281 |
| Organism |
Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Protostomia
Ecdysozoa
Panarthropoda
Arthropoda
Mandibulata
Pancrustacea
Hexapoda
Insecta
Dicondylia
Pterygota (winged insects)
Neoptera
Endopterygota
Diptera
Brachycera
Muscomorpha
Eremoneura
Cyclorrhapha
Schizophora
Acalyptratae
Ephydroidea
Drosophilidae (pomace flies)
Drosophilinae
Drosophilini
Drosophila (fruit flies)
Sophophora
melanogaster group
melanogaster subgroup
Drosophila melanogaster (Fruit fly)
|
| Enzyme Sequence |
MDLRKHLGLLTLLLVAVFAFYGRPADACSCMPSHPQTHFAQADYVVQLRVLRKSDTIEPGRTTYKVHIKRTYKATSEARRMLRDGRLSTPQDDAMCGINLDLGKVYIVAGRMPTLNICSYYKEYTRMTITERHGFSGGYAKATNCTVTPCFGERCFKGRNYADTCKWSPFGKCETNYSACMPHKVQTVNGVISRCRWRRTQLYRKCMSNP |
| Enzyme Length |
210 |
| Uniprot Accession Number |
Q9VH14 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Metalloproteinase inhibitor that acts on both matrix metalloproteinases Mmp1 and Mmp2 in vitro (PubMed:14567681). Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor (By similarity). Required for wing maturation which is the final step in morphogenesis of the adult fly (PubMed:16962574). Involved in the negative regulation of developmental tissue invasion for imaginal disk eversion during metamorphosis by inhibiting Mmp-mediated basement membrane (BM) degradation (PubMed:17301221). Required for oogenesis and for the long-term maintainance of germarial structure and shape in the adult ovaries. Required for maintaining composition and biophysical properties of the extracellular matrix (ECM), and for the normal organization and cyst production of the germline stem cell (GSC) niche (PubMed:26808525). {ECO:0000250|UniProtKB:P16035, ECO:0000269|PubMed:14567681, ECO:0000269|PubMed:16962574, ECO:0000269|PubMed:17301221, ECO:0000269|PubMed:26808525}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Chain (1); Disulfide bond (5); Domain (1); Metal binding (1); Region (2); Sequence conflict (1); Signal peptide (1) |
| Keywords |
Developmental protein;Differentiation;Direct protein sequencing;Disulfide bond;Metal-binding;Metalloenzyme inhibitor;Metalloprotease inhibitor;Oogenesis;Protease inhibitor;Reference proteome;Secreted;Signal;Zinc |
| Interact With |
|
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:14567681}. |
| Modified Residue |
|
| Post Translational Modification |
|
| Signal Peptide |
SIGNAL 1..27; /evidence=ECO:0000269|PubMed:14567681 |
| Structure 3D |
|
| Cross Reference PDB |
- |
| Mapped Pubmed ID |
10578110;
12530966;
12593803;
15255973;
15780979;
15944190;
16210248;
16369566;
16815999;
17082773;
17239363;
18045838;
19196956;
19751719;
20152186;
20220848;
20371351;
20404112;
20495554;
21074052;
21177962;
21343364;
21399659;
21669931;
21978772;
21979943;
22078297;
22262460;
22265916;
22438831;
22498775;
22685676;
22782723;
22870402;
23071443;
23570874;
23583979;
23944235;
24475130;
24509725;
24685612;
24859129;
25267296;
25312911;
25344916;
25520167;
25609699;
25695427;
26202798;
26440503;
26473732;
26603384;
26690827;
27127457;
27172210;
28192468;
28300207;
28537903;
28550016;
28576972;
28825401;
29056683;
29118190;
29256860;
29411505;
29853618;
29974861;
30246928;
31188709;
31195239;
31577228;
31722958;
32098783;
32105665;
32122911;
32598400;
32649911;
32788209;
33077027;
33159074;
33322177;
33377870;
33578809;
34496252;
|
| Motif |
|
| Gene Encoded By |
|
| Mass |
23,980 |
| Kinetics |
|
| Metal Binding |
METAL 28; /note=Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partner; /evidence=ECO:0000250|UniProtKB:P01033 |
| Rhea ID |
|
| Cross Reference Brenda |
|