| IED ID | IndEnz0002011081 |
| Enzyme Type ID | protease011081 |
| Protein Name |
Trypsin EC 3.4.21.4 |
| Gene Name | |
| Organism | Sus scrofa (Pig) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Suina Suidae (pigs) Sus Sus scrofa (Pig) |
| Enzyme Sequence | FPTDDDDKIVGGYTCAANSIPYQVSLNSGSHFCGGSLINSQWVVSAAHCYKSRIQVRLGEHNIDVLEGNEQFINAAKIITHPNFNGNTLDNDIMLIKLSSPATLNSRVATVSLPRSCAAAGTECLISGWGNTKSSGSSYPSLLQCLKAPVLSDSSCKSSYPGQITGNMICVGFLEGGKDSCQGDSGGPVVCNGQLQGIVSWGYGCAQKNKPGVYTKVCNYVNWIQQTIAAN |
| Enzyme Length | 231 |
| Uniprot Accession Number | P00761 |
| Absorption | |
| Active Site | ACT_SITE 48; /note=Charge relay system; ACT_SITE 92; /note=Charge relay system; ACT_SITE 185; /note=Charge relay system |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; |
| DNA Binding | |
| EC Number | 3.4.21.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (16); Chain (1); Disulfide bond (6); Domain (1); Helix (4); Metal binding (4); Natural variant (1); Propeptide (1); Site (1); Turn (3) |
| Keywords | 3D-structure;Calcium;Digestion;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (36) |
| Cross Reference PDB | 1AKS; 1AN1; 1AVW; 1AVX; 1C9P; 1EJA; 1EPT; 1FMG; 1FN6; 1FNI; 1H9H; 1H9I; 1LDT; 1MCT; 1QQU; 1S5S; 1S6F; 1S6H; 1S81; 1S82; 1S83; 1S84; 1S85; 1TFX; 1TX6; 1UHB; 1V6D; 1YF4; 1Z7K; 2A31; 2A32; 3MYW; 4AN7; 4DOQ; 5XWJ; 5XWL; |
| Mapped Pubmed ID | 10329799; 10409654; 10441159; 10512718; 10547291; 10561533; 11679713; 14672690; 15005618; 15106996; 15299934; 15381428; 15842169; 15854654; 16131759; 16536459; 23094997; 24121345; 2622910; 29486250; 8444191; 9199408; 9466914; |
| Motif | |
| Gene Encoded By | |
| Mass | 24,409 |
| Kinetics | |
| Metal Binding | METAL 60; /note=Calcium; METAL 62; /note=Calcium; via carbonyl oxygen; METAL 65; /note=Calcium; via carbonyl oxygen; METAL 70; /note=Calcium |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.4; |