| IED ID | IndEnz0002011083 |
| Enzyme Type ID | protease011083 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 36 EC 3.4.19.12 Deubiquitinating enzyme 36 Protein scrawny Ubiquitin thioesterase 36 Ubiquitin-specific-processing protease 36 |
| Gene Name | Usp36 scny GK17299 |
| Organism | Drosophila willistoni (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora willistoni group willistoni subgroup Drosophila willistoni (Fruit fly) |
| Enzyme Sequence | MPVSLAVCETTTTTTANVVNAALRESLLGGSRSGAAVAGSGSASSLISGAGGAGASAANDDDSSSTATANTNLQNQIVASAKRVLLAKIEYEEVENYNDSVLDNLKTKYVVIKSPTPAAAAAATNNGNSNSAGSKILGANGHDNRQKQQNANGGCSTPTTTTTQTSSSSTSSSSIEHNNNPNELPKPKRVLYQRENIRIGWKQSERKWQIGAGMINVGNTCYLNSTLQALFHIPALANWLNSEQSHLENCNIGGESGGGGGGGGGNNGGFCIICAMAKTLQSTQSNASAIRPYHIYSKLKQICKHMVIGRQEDAHEFLRFLVEAMEKAYLMRYRNYKELDQLVKETTPLNQIFGGYLRSEVRCLSCNHVSITFQHFQDLLLDIRKSDTLDEAFDGYFSRERLEDMGYKCEGCKKKVSATKQFSLQRAPITLCIQLKRFSMIGNKLTKQISFKPRIDLSRFAARSPTAQGQLPLTYRLVSLVTHLGVSQHCGHYTAIGLTESGSYYNFDDSYVRPIAMQSVCSTNSYIMFYELDLNQPLATPANKLNGLRQLSNGHHHHQQQQQQHQQQQQQQPTVVATIASSPMATTRFIGPQLPPGGLNGYAMTTTTTATNNTTNGHSQKTAIQFKPQQNGILTVGSGKFQESGQQKSPLVGTNHKNEAPAVAPNANANANGKSSSNPNTNTTINTNTNNGNSNNNNTNATTANNSNKLNQQQQQYLPMSSSDEEDSDEEKMKRPTTTTPQLPSMPKMDGGGDEKPKTLTLTPTTTPTASTPTVSNPLKRLVPYESASEEEESSSGTPSSSTPTTTTTAAAAAASSPMQATAAATLPPPPTPTNARKRSLPDHHHHHPHHHVMVNGHGKSPKPASMPPATNFNSSSSKQKTDAIDEIFKSLNNFNKKRINNKNQKHNEGDEEEDDEETLEKETNNSSRLVSSSTNTSPTTNGWKQSQIVSSSSSNSKNVSTSAAAAAATTSSSTSTSAPPSPKTPPSPAVINSKTGLWKVTRNLDDDDDEEEEDEDDEEHIAPTPPPVVAKTHKNPFSSQQKPTPSPSTEAAPKRQKLFNGTSSSTPHVGNGYQSEPSTPNGGGSGSGSNGCLNELLKQSHRGYGSSSVLSWNGKQTELDKEPFELVCAKRIAVDHGDHDDGGGGDDGGGVGVVTTTTTTTTTTKNTTKTLTADAQEQRQRDLDDDEENEMDRGRQRKVKTATANGKSSGNSNNTTPGYNPFQEYESQKRWHNKSSNGPPRFYTQHASSSYRSNFHQRNKFKCNRGGNGGGGSGGISKFDHRHGLQRHLAAGGGFPRRPNANQQQQQQQS |
| Enzyme Length | 1311 |
| Uniprot Accession Number | B4MLR8 |
| Absorption | |
| Active Site | ACT_SITE 221; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093"; ACT_SITE 492; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Controls selective autophagy activation by ubiquitinated proteins. {ECO:0000250|UniProtKB:Q9VRP5}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (15); Domain (1); Modified residue (9); Region (5) |
| Keywords | Hydrolase;Nucleus;Phosphoprotein;Protease;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}. |
| Modified Residue | MOD_RES 581; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 767; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 787; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 789; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 982; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 985; /note=Phosphothreonine; /evidence=ECO:0000250; MOD_RES 988; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 1047; /note=Phosphoserine; /evidence=ECO:0000250; MOD_RES 1050; /note=Phosphothreonine; /evidence=ECO:0000250 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 141,002 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |