| IED ID | IndEnz0002011103 |
| Enzyme Type ID | protease011103 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 16 EC 3.4.19.12 Deubiquitinating enzyme 16 Ubiquitin thioesterase 16 Ubiquitin-specific-processing protease 16 |
| Gene Name | usp16 |
| Organism | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amphibia Batrachia Anura Pipoidea Pipidae Xenopodinae Xenopus Silurana Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
| Enzyme Sequence | MVKKRGKNLPAQDSLDAEPVCKHLRKALDEGSVKKALVNVEWTVCQECQADNKEKNNSDDELVEDPSVWLCLKCGHRGCGRNSASQHALNHYNTPRSEPHCLVLSVDMWSAWCYLCDNEVPYNRSSRLGQLVDYLQRKAKAKSKSTDSAALDEEVKAEIVAENEVKIEDQEEKPKGQAKWDKASSTQNNSTEPTVKGLSNLGNTCFFNAVMQNLSQTPAVRELLNEAKTLKKPVTVPLPDSSSPTNVEVHLEQQPGPLTLAMWQFLTEMQETKKGVVTPKEVFSQVCKKAIRFKGYQQQDSQELLRYLLDGMRGEEIQRVSLAMSKSLQNTLDEEEIKKIVKDSEKRRTIPNFVDHLFGGELTSTIMCEECHTVSLVHEPFLDLSLPVLDDVIVKKNSQKSSAPAPERKEEEENDDGYIKERDEASPGASKHLQKKAKKAAKKQAKNQRRQLKMQGKTVLLTDVAKQECSEDEEEIAPNNTESEANTRPDDEVPIADGLNTMKSDLSALENGSETIESAMERVTEDTDLDTSGHNTESVEMNAMELVRNMENNNNNNTDVNKTLERTEGSGVDSMEATAAVDNGNADTVCVDDTEAANGLLDCSAASMDNELTNSLNRLKLSSDIEPTQVEIEILPDQQQPHTQIYEVINEDPKTAFSTLSERKDLPLDGYSVLSCLYQFTHKETLTGNNKLLCNVCTRKQASRLNNSNKGEKTFVYTNAKKQMLVSDPSPILTLHLKRFQQNGFNLRKINRHIKFPEVLDLAPFCTSKCKNIPAGESRLLYSLYGVIEHSGSMRSGHYTAFVKLRRPNQQLCEMVLKGVIPEVSGSEPGQGSWYHISDSHVQAVSLSRVLSSQAYLLFYERML |
| Enzyme Length | 864 |
| Uniprot Accession Number | Q0VA64 |
| Absorption | |
| Active Site | ACT_SITE 205; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_03062; ACT_SITE 798; /note=Proton acceptor; /evidence=ECO:0000255|HAMAP-Rule:MF_03062 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000255|HAMAP-Rule:MF_03062}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. {ECO:0000255|HAMAP-Rule:MF_03062}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (4); Domain (1); Metal binding (12); Region (2); Sequence conflict (9); Zinc finger (1) |
| Keywords | Activator;Cell cycle;Cell division;Chromatin regulator;Hydrolase;Metal-binding;Mitosis;Nucleus;Protease;Reference proteome;Thiol protease;Transcription;Transcription regulation;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03062}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 96,666 |
| Kinetics | |
| Metal Binding | METAL 21; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 23; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 45; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 48; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 71; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 74; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 79; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 87; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 91; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 100; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 113; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502; METAL 116; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00502 |
| Rhea ID | |
| Cross Reference Brenda |