| IED ID | IndEnz0002011113 |
| Enzyme Type ID | protease011113 |
| Protein Name |
DNA-dependent metalloprotease WSS1 homolog 2 EC 3.4.24.- DNA damage response protein WSS1 homolog 2 |
| Gene Name | wss2 wss1b SPCC1442.07c |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MELKFSCRGNVIALSFNENDTVLDAKEKLGQEIDVSPSLIKLLYKGNLSDDSHLQDVVKNESKIMCLIRQDKDIVNQAISQLKVPDYSTNTYSLKPKKPHTTPKPASIYTFNELVVLDYPHKDRALRYLERLRDDTGIKKIMDSHRWTVPLLSEMDPAEHTRHDSKTLGLNHNQGAHIELRLRTDRYDGFRDYKTVKSTLIHELTHNVHGEHDSSFWELFRQLTKEADAADLLGKPGSYVSDRASYTPQQDNDDEDQKNHRRDLLLAAAERRKQSGSKVQKE |
| Enzyme Length | 282 |
| Uniprot Accession Number | O94580 |
| Absorption | |
| Active Site | ACT_SITE 203; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Metalloendopeptidase that acts selectively on DNA-binding proteins. DNA is needed to bring the protease and substrates together to enable proteolysis. Involved in the repair of toxic DNA-protein cross-links (DPCs) such as covalently trapped topoisomerase 1 (TOP1) adducts on DNA lesions or DPCs induced by reactive compounds such as formaldehyde. {ECO:0000250|UniProtKB:P38838}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Beta strand (6); Chain (1); Compositional bias (1); Domain (2); Helix (4); Metal binding (3); Region (1); Turn (2) |
| Keywords | 3D-structure;Cytoplasm;DNA damage;DNA repair;Hydrolase;Metal-binding;Metalloprotease;Nucleus;Protease;Reference proteome;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus {ECO:0000269|PubMed:16823372}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 5JIG; 5LN5; |
| Mapped Pubmed ID | 20473289; 21247416; 23697806; 30647105; 32034465; |
| Motif | |
| Gene Encoded By | |
| Mass | 32,464 |
| Kinetics | |
| Metal Binding | METAL 202; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10095, ECO:0000305|PubMed:27871365, ECO:0007744|PDB:5JIG, ECO:0007744|PDB:5LN5"; METAL 206; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10095, ECO:0000305|PubMed:27871365, ECO:0007744|PDB:5JIG, ECO:0007744|PDB:5LN5"; METAL 212; /note="Zinc; catalytic"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10095, ECO:0000305|PubMed:27871365, ECO:0007744|PDB:5JIG, ECO:0007744|PDB:5LN5" |
| Rhea ID | |
| Cross Reference Brenda |