| IED ID | IndEnz0002011141 |
| Enzyme Type ID | protease011141 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 32 EC 3.4.19.12 |
| Gene Name | Usp32 Usp15 CG8334 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MLYAACGGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVRNVNLAPFESVSLFAQSERVNFEQFQDWIIKHRNATVLSKWLLSDNCVSLTSELETPTFYQSLAGVTHLEEKDIGDLEKEFWRLKNTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQYKDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQYTQHTTHTCDYCKRTASQRTAVDEALVCDESFNTHSLEQHDSYSLGSGTGSASGSGSASSGISAGRHCGPVRPGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELVPKSLWKALNRWYGDNLPLPRQVIQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSAQDSLSSLSTLQTSSHRASSRVLCNGHVSGLDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKVSRCFGKRECMPHSLFCFKESLILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGENSMESSLTEPIPLADLEPVSSRNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQPDQITTSQPETSSGVYSRRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQWYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQETILRHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATASPPPTGNILRQSKTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGGHYISYASNATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEGRTLPNTASVPLEVDETEGELKKLCSIS |
| Enzyme Length | 1715 |
| Uniprot Accession Number | M9PD06 |
| Absorption | |
| Active Site | ACT_SITE 686; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01035; ACT_SITE 1633; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01035 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | CA_BIND 174..185; /note=1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448; CA_BIND 210..221; /note=2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00448 |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000250|UniProtKB:Q8NFA0}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinating enzyme that acts as an inhibitor of mitophagy probably by counteracting the action of park. Possibly functions by hydrolyzing ubiquitin attached by park on target proteins, thereby reducing park's ability to drive mitophagy. {ECO:0000269|PubMed:24852371}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (2); Calcium binding (2); Chain (1); Compositional bias (5); Domain (4); Region (3) |
| Keywords | Alternative splicing;Calcium;Hydrolase;Phosphoprotein;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20220848; 20371351; 20439780; 21074052; 22937016; 23071443; 23275879; 23899565; 25027767; 26120032; 26512413; 26550693; 26917723; 27768896; 28621429; 29809156; 33988679; |
| Motif | |
| Gene Encoded By | |
| Mass | 191,844 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |