| IED ID | IndEnz0002011165 |
| Enzyme Type ID | protease011165 |
| Protein Name |
Ubiquitin carboxyl-terminal hydrolase 46 EC 3.4.19.12 Deubiquitinating enzyme 46 Ubiquitin thioesterase 46 Ubiquitin-specific-processing protease 46 |
| Gene Name | USP46 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MTVRNIASICNMGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQKKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSSDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE |
| Enzyme Length | 366 |
| Uniprot Accession Number | P62068 |
| Absorption | |
| Active Site | ACT_SITE 44; /note="Nucleophile"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093, ECO:0000269|PubMed:26388029, ECO:0000305|PubMed:22043315"; ACT_SITE 313; /note="Proton acceptor"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10092, ECO:0000255|PROSITE-ProRule:PRU10093" |
| Activity Regulation | ACTIVITY REGULATION: Activated by interaction with WDR48. {ECO:0000269|PubMed:26388029}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000269|PubMed:14715245, ECO:0000269|PubMed:19075014, ECO:0000269|PubMed:26388029}; |
| DNA Binding | |
| EC Number | 3.4.19.12 |
| Enzyme Function | FUNCTION: Deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. May act by mediating the deubiquitination of GAD1/GAD67 (By similarity). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed:19075014, PubMed:26388029). Not involved in deubiquitination of monoubiquitinated FANCD2 (PubMed:19075014). {ECO:0000250|UniProtKB:P62069, ECO:0000269|PubMed:19075014, ECO:0000269|PubMed:26388029}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (4); Beta strand (22); Chain (1); Domain (1); Helix (10); Metal binding (4); Mutagenesis (1); Natural variant (1); Sequence conflict (3); Turn (3) |
| Keywords | 3D-structure;Alternative splicing;Behavior;Hydrolase;Metal-binding;Protease;Reference proteome;Thiol protease;Ubl conjugation pathway;Zinc |
| Interact With | P54253; Q8TBZ3 |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 5CVM; 5CVN; 5CVO; 5L8H; 6JLQ; |
| Mapped Pubmed ID | 19260141; 19615732; 20111060; 21663972; 22391563; 25814554; 25855980; 30415951; 31542232; 31632687; 33029497; 33576437; 34029571; |
| Motif | |
| Gene Encoded By | |
| Mass | 42,442 |
| Kinetics | |
| Metal Binding | METAL 182; /note="Zinc"; /evidence="ECO:0007744|PDB:5CVM, ECO:0007744|PDB:5CVN, ECO:0007744|PDB:5CVO, ECO:0007744|PDB:5L8H"; METAL 185; /note="Zinc"; /evidence="ECO:0007744|PDB:5CVM, ECO:0007744|PDB:5CVN, ECO:0007744|PDB:5CVO, ECO:0007744|PDB:5L8H"; METAL 229; /note="Zinc"; /evidence="ECO:0007744|PDB:5CVM, ECO:0007744|PDB:5CVN, ECO:0007744|PDB:5CVO, ECO:0007744|PDB:5L8H"; METAL 232; /note="Zinc"; /evidence="ECO:0007744|PDB:5CVM, ECO:0007744|PDB:5CVN, ECO:0007744|PDB:5CVO, ECO:0007744|PDB:5L8H" |
| Rhea ID | |
| Cross Reference Brenda |