| IED ID | IndEnz0002011185 |
| Enzyme Type ID | protease011185 |
| Protein Name |
Venom metalloproteinase 3 EpMP3 |
| Gene Name | |
| Organism | Eulophus pennicornis (Parasitoid wasp) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Hymenoptera Apocrita (wasps ants and bees) Parasitoida Chalcidoidea Eulophidae Eulophinae Eulophus Eulophus pennicornis (Parasitoid wasp) |
| Enzyme Sequence | QYSESQESGHNRNAPDKELTTEEFQLIFHQSQTVDIEYDFINITTEMIETERKVSFTIDGKEYHLSLTPAASQSVLPYGTKIKSAIWWTDNDTHIHEEDYSDERWDSRAIYENLEIMATILVRTENGTSYYDGVFGEGIAMKVVRSLPGRLMNIYGANYHFVYDSNGSVYDVVLNGQDEPAVPADMAVNNFYPKLLVLVDYSLFKIFNENFEETVKYLTIFWNAVNLRFRPVQHPKVNIIITGIVIAKNEAAFQHVYRARYSKNSKLVHTGRVIDNGRYFFGTNFDPYYDNYDASFTMASMDDPTGKGGATVIGGICSSSNNIAYIRDVGSYSGVKVATHELGHLLNGQHDSDTTCSEKINDNIYTIMAKQGSTKASKFVWSSCTLTAFANFSKTTSAACLKDTYRK |
| Enzyme Length | 407 |
| Uniprot Accession Number | B5AJT4 |
| Absorption | |
| Active Site | ACT_SITE 341; /evidence="ECO:0000255|PROSITE-ProRule:PRU00276, ECO:0000255|PROSITE-ProRule:PRU10095" |
| Activity Regulation | ACTIVITY REGULATION: The gelatinase activity is inhibited by EDTA. {ECO:0000269|PubMed:19320760}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: The recombinant protein has gelatinase activity. In vivo, injection of this recombinant into fifth instar L.oleracea (host) larvae results in partial insect mortality associated with the molt to sixth instar, with surviving insects showing retarded development and growth. {ECO:0000269|PubMed:19320760}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (2); Domain (1); Glycosylation (5); Metal binding (3) |
| Keywords | Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Toxin;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 46,007 |
| Kinetics | |
| Metal Binding | METAL 340; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 344; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 350; /note=Zinc; catalytic; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |