| IED ID | IndEnz0002011189 |
| Enzyme Type ID | protease011189 |
| Protein Name |
Vacuolar-processing enzyme gamma-isozyme EC 3.4.22.34 Asparaginyl endopeptidase gamma-VPE Gamma-VPE |
| Gene Name | At4g32940 F26P21.60 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MATTMTRVSVGVVLFVLLVSLVAVSAARSGPDDVIKLPSQASRFFRPAENDDDSNSGTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTIINSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYELVKRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFADANSLKPPSRVTNQRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGISRGPEVLNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQMEEAASQACTTLPTGPWSSLNRGFSA |
| Enzyme Length | 494 |
| Uniprot Accession Number | Q39119 |
| Absorption | |
| Active Site | ACT_SITE 177; /evidence=ECO:0000255; ACT_SITE 219; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.; EC=3.4.22.34; Evidence={ECO:0000250|UniProtKB:Q84LM2}; |
| DNA Binding | |
| EC Number | 3.4.22.34 |
| Enzyme Function | FUNCTION: Asparagine-specific endopeptidase involved in the processing of vacuolar seed protein precursors into the mature forms (PubMed:10417725). Probably involved in post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds (PubMed:14688293). {ECO:0000269|PubMed:10417725, ECO:0000269|PubMed:14688293}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (11); Chain (1); Erroneous initiation (1); Helix (18); Signal peptide (1); Site (1); Turn (3) |
| Keywords | 3D-structure;Hydrolase;Protease;Reference proteome;Signal;Thiol protease;Vacuole |
| Interact With | |
| Induction | INDUCTION: By senescence, wounding, jasmonic acid (JA), ethylene and salicylic acid (SA). {ECO:0000269|PubMed:10417725}. |
| Subcellular Location | SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:8589932}. Lytic vacuole {ECO:0000269|PubMed:10417725}. Note=Localized in lytic vacuoles of wounded leaves. {ECO:0000269|PubMed:10417725}. |
| Modified Residue | |
| Post Translational Modification | PTM: Auto-catalytic activation. {ECO:0000250|UniProtKB:Q84LM2}. |
| Signal Peptide | SIGNAL 1..29; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 5NIJ; |
| Mapped Pubmed ID | 11867693; 12799370; 14600834; 16043487; 18650403; 18775970; 22497243; 22822203; 28627464; 29453229; 29628443; 31279878; 31507623; |
| Motif | |
| Gene Encoded By | |
| Mass | 54,336 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.34; |